缝隙连接蛋白43在大鼠肺动脉高压及波生坦干预后表达的变化  

Expression of Connexin43 in Rats With Pulmonary Arterial Hypertension Induced by Monocrotaline and the Effect of Bosentan Treated Expression

作　　者：谭晓燕[1] 何建国[1] 

机构地区：[1]中国科学院北京协和医学院心血管病研究所阜外心血管病医院心内科,北京市100037

出　　处：《中国循环杂志》2010年第1期65-68,共4页Chinese Circulation Journal

基　　金：高等学校博士学科点专项科研基金资助课题(20060023042)

摘　　要：目的:研究肺小动脉内皮细胞间缝隙连接蛋白(Cx)43在大鼠肺动脉高压及波生坦(bosentan)干预后表达的变化。方法:10只SD大鼠为正常对照组(n=10),不做任何处理。57只SD大鼠通过大鼠腹腔内注射野百合碱(MCT,60mg/kg)两周后,随即将大鼠分成2组,具体分组如下:肺动脉高压大鼠+安慰剂组(n=35),肺动脉高压大鼠+bosen-tan组(n=22)。通过胃管予以安慰剂或双通道内皮素受体拮抗剂[bosentan,300mg/(kg.d)]治疗,每天1次,共计3周。免疫荧光的方法测定Cx43和Cx37在肺小动脉中层平滑肌细胞间和内皮细胞间表达的变化。结果:与正常对照组比,肺动脉高压大鼠+安慰剂组的肺小动脉中层平滑肌厚度和血管外周直径的比值(WT%)、肺动脉平均压均明显增加(P均<0.01),肺动脉高压大鼠+bosentan组的WT%较肺动脉高压大鼠+安慰剂组显著减小(P<0.01)。在正常对照组的肺小动脉内皮细胞间未检测到Cx43,而在肺动脉高压大鼠+安慰剂组和肺动脉高压大鼠+bosentan组,内皮细胞间均可见Cx43表达,其表达量在肺动脉高压大鼠+bosentan组显著少于肺动脉高压大鼠+安慰剂组(P<0.01)。3组肺小动脉内皮细胞间均可见Cx37的表达,但3组间的表达量差异无统计学意义。结论:在肺动脉高压中肺小动脉内皮细胞间出现了Cx43的异常表达,经bosentan治疗后Cx43的表达水平显著下降。Objective：To study the expression of connexin43 （Cx43） in endothelial cells of small pulmonary artery in rats with pulmona- ry arterial hypertension （PAH） induced by monocrotaline and the effect of bosentan treated expression. Methods：A total of 67 experimental male rats were divided into three groups. Control group （n = 10）, and the other 57 rats were injected with monocrotaline （MCT 60 mg/kg） for 2 weeks to induce PAH model,and then,the PAH rats were divided into two groups ,PAH ＋ vehicle group （ n = 35 ） , and PAH ＋ bosentan group （ n = 22 ）. The changes of Cx43 and Cx37 expression in endothelial cells and smooth muscle cells of small pulmonary arteries were examined by immuno-fluorescence assay. Results： Compared with Control group, the mean pulmonary arterial pressure and the percent medial wall thickness （WT%） in small pulmonary arteries were markedly increased in PAH ＋ vehicle group （ P 〈 0. 01 ）. Compared with PAH ＋ vehicle group, the （ WT% ） was significantly decreased in PAH ＋ bosentan group （ P 〈 0. 01 ）. Cx43 was undetectable in endothelial cells in Control group, but it was expressed in both PAH ＋ vehicle and PAH ＋ bosentan groups. The expression of Cx43 was significantly lower in PAH ＋ bosentan group than that in PAH ＋ vehicle group. Cx37 was found in all three groups, and the expression was no statistic meaning among different group. Conclusion ： The abnormal expression of Cx43 was observed in endothelial cells of small pulmonary artery in PAH rats, and this expression was significantly decreased by bosentan treatment.

关 键 词：肺动脉高压 缝隙连接蛋白 平滑肌细胞 内皮细胞 

分 类 号：R318[医药卫生—生物医学工程]