不同规模污水处理系统中微生物群落结构  

Microbial communities in different size wastewater treatment systems

作　　者：王晓慧[1] 文湘华[1] 马娜[2] 丁鵾 许美兰[1] 杨宁宁[1] 

机构地区：[1]清华大学环境科学与工程系,北京100084 [2]中国矿业大学化学与环境工程学院,北京100083

出　　处：《清华大学学报（自然科学版）》2010年第3期411-414,共4页Journal of Tsinghua University(Science and Technology)

摘　　要：为了研究处理规模对污水处理系统中微生物群落结构的影响,该文采用针对氨氧化菌(AOB)功能基因氨单加氧酶(amoA),及针对细菌16S rRNA基因的末端限制性片段长度多态性技术(T-RFLP),分析了北京市一个实际污水处理系统和一个中试反应器中AOB及细菌的群落结构,并探讨了处理规模对微生物群落结构的影响。T-RFLP指纹图谱表明:2个污水处理系统中AOB的优势末端限制性片段(T-RF)均为291和354 bp,细菌的优势T-RF为106、115、117、166、423、455、468、471、482、739、777和782bp等。T-RFLP指纹图谱分析表明:处理规模对系统中AOB群落结构没有显著影响,而对系统中细菌的多样性有一些影响。2个污水处理系统中优势AOB均属于Nitrosomonas europaeacluster和Nitrosomonas oligotrophaculster。Communities of ammonia oxidizing bacteria （AOB） and bacteria in activated sludges collected from a full-scale wastewater treatment system and a pilot-scale bioreactor in Beijing were investigated to evaluate the effect of treatment plant size on the microbial communities. The investigation used terminal restriction fragment length polymorphism （T-RFLP） to target whoa in AOB and 16S rRNA genes in the sludge. The T RFLP fingerprints showed that the dominant terminal restriction fragments （T-RFs） of AOB from these two systems were 291, 354 bp, and the dominant T-RFs of the bacteria were 106. 115, 117, 166, 423, 455, 468, 471, 482, 739, 777, and 782 bp. The T-RFLP profiles indicated that the size of the wastewater treatment system had no significant impact on the AOB communities, but slightly affected the bacterial diversity. The dominant AOB in these two systems were bacterial members of the Nitrosomonas europaea and Nitrosomonas oligotropha clusters.

关 键 词：氨氧化菌(AOB) 细菌 末端限制性片段长度多态 性技术(T-RFLP) 处理规模 

分 类 号：Q939.1[生物学—微生物学]