同种异体骨髓基质干细胞移植对兔激素性股骨头缺血性坏死血清肿瘤坏死因子α的影响  

Effects of allogenic marrow stromal cells transplantation on the TNF-α in rabbits with steroid-induced avascular necrosis of femoral head

作　　者：陈宣维[1] 林文平[1] 林建华[1] 

机构地区：[1]福建医科大学附属第一医院骨科,350001

出　　处：《中国实用医药》2010年第6期4-5,共2页China Practical Medicine

基　　金：福建省科技计划项目(项目编号:2005y014)

摘　　要：目的研究同种异体骨髓基质干细胞(MSC)移植对兔激素性股骨头缺血性坏死(SAN-FH)血清肿瘤坏死因子α(TNF-α)的影响。方法将45只健康新西兰兔随机分为三组:空白组、每组各15只。模型组和治疗组,模型组和治疗组,第1、3周马血清10ml/kg耳缘静脉注射,于第5周腹腔注射醋酸泼尼松龙7.5mg/kg,1次/d,连续3d;空白组,第1、3周生理盐水10ml/kg兔耳缘静脉注射,于第5周腹腔注射生理盐水1ml,1次/d,连续3d。治疗组于第6周行MSC移植。第10周测血清中TNF-α水平。结果治疗组兔血清TNF-α水平较模型组明显偏低(P<0.01)。结论MSC移植使兔SANFH模型血清TNF-α水平降低。Objective To study the effects of allogenic marrow stromal cells transplantation on the TNF-α in rabbits with steroid-induced avascular necrosis of femoral head. Methods Forty-five healthy New Zealand white rabbits were randomly divided into control group, model group and treatment group with 15 in each group. Model group and treatment group were injected with horse serum and prednisone. The model group was given hormal saline treatment group was transplanted of allogenie marrow stromal cells at 6 weeks. The level of TNF-α in serum of the rabbits of the three groups was examined at 10 weeks. Results The level of TNF-α in rabbits serum of treatment group was lower than that of model group （ P 〈 0. 01 ）. Conclusions Marrow stromal cells transplantation can decrease the level of TNF-α on steroid-induced avascular necrosis of femoral head in rabbits.

关 键 词：骨髓基质干细胞 股骨头坏死 肿瘤坏死因子 

分 类 号：R681.8[医药卫生—骨科学]