白术和黄芪不同提取部位对小肠上皮细胞增殖的影响  

Effects of Different Extract Parts from Rhizoma Atractylodis Macrocephalae and Radix Astragali on Proliferation of Intestinal Epithelial Cells

作　　者：胡灿[1] 李茹柳[1] 莫全毅[1] 王治平[2] 林传权[1] 陈炜璇[1] 陈蔚文[1,3] 

机构地区：[1]广州中医药大学脾胃研究所,广州510405 [2]广州中医药大学中药学院,广州510006 [3]上海市高校中医内科学E-研究院,上海中医药大学,上海201203

出　　处：《中药新药与临床药理》2010年第2期156-160,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(30772753);广东省自然科学基金项目(7300343);上海市教育委员会E-研究院建设计划项目(E03008)

摘　　要：目的观察白术和黄芪的不同提取部位对小肠上皮细胞(IEC-6)增殖的影响。方法以系统溶剂分离法分别提取白术和黄芪的石油醚、乙酸乙酯、正丁醇、醇沉上清液和多糖部位;另将白术和黄芪以乙醇提取,D101大孔树脂吸附法得到40%和70%乙醇洗脱部位。MTT法检测白术和黄芪正丁醇部位、醇沉上清液、多糖部位、40%和70%醇洗脱液对IEC-6细胞增殖的作用。结果初步发现黄芪正丁醇部位、醇沉上清部位、糖复合物、40%和70%醇洗脱部位加药24h能促进细胞增殖;白术40%乙醇洗脱液部位加药24h能促进细胞增殖,白术正丁醇部位、糖复合物、40%和70%乙醇洗脱液部位加药48h能促进细胞增殖。结论白术和黄芪正丁醇部位、多糖部位、40%和70%醇洗脱液在加药后不同时间对IEC-6细胞有不同程度的促进增殖作用;采用系统溶剂分离法和大孔树脂吸附法提取白术和黄芪不同部位,可以用于细胞增殖实验,为进一步开展白术和黄芪在细胞水平上的药理研究提供参考。Objective To study effects of different extract parts from Rhizoma Atractylodis Macrocephalae （RAM）and Radix Astragali（RA）on proliferation of intestinal epithelial cells. Methods Polysaccharides, petroleum ether extracts, acetic ether extracts, N-butanol extracts and ethanol supernate from RAM and RA have gotten by means of systematic solvent extraction. Total saponins have obtained by alcohol （at the concentration of 40 % and 70 %） reflux extraction and purified by macroporous resin D-101. We observed the influence of N-butanol extracts, ethanol supernate, polysaccharides, and 40 % and 70 % ethanol eluants on the proliferation of intestinal epithelial cells by MTT assay. Results N-butanol extracts, ethanol supernate, polysaccharide, and 40 % and 70 % ethanol eluants from RA, and the sample from RAM eluted by 40 % ethanol promoted the proliferation of intestinal epithelial cells after treatment for 24 hours. After treatment for 48 hours, N-butanol extracts, polysaccharide, and 40 % and 70 % ethanol eluants from RAM promoted the proliferation of intestinal epithelial cells. Conclusion N-butanol extracts, ethanol supernate, polysaccharide, and 40 % and 70 % ethanol eluants from RAM and RA can be obtained by means of systematic sol- vent extraction and macroporous resin adsorption method, and they exert different degrees of effect on the prolifera- tion of intestinal epithelial cells, which will provide evidence for the research of pharmacological mechanism of RAM and RA.

关 键 词：白术 黄芪 提取部位 细胞增殖 

分 类 号：R285.5[医药卫生—中药学]