紫外分光光度法测定明党参中总香豆素类成分的含量  

Determination of Total Coumarin in Changium smyrnioides Wolff.

作　　者：顾源远[1] 陈建伟[1] 

机构地区：[1]南京中医药大学,江苏南京210046

出　　处：《现代中药研究与实践》2010年第2期58-60,共3页Research and Practice on Chinese Medicines

摘　　要：目的建立明党参药材中总香豆素含量测定方法。方法采用紫外分光光度法,以珊瑚菜内酯为对照品,检测波长为268 nm。结果在2.65～7.94μg/mL质量浓度范围内,对照品珊瑚菜内酯的吸光度和浓度线性关系良好,R^2=0.999 0。平均回收率为103.84%,RSD=1.75%(n=6)。结论该法简便,可靠,为有效控制明党参药材质量奠定了一定的基础。Objective To establish a method for determination of the total coumarin in Csmyrnioides Wolff.. Methods With phellopterin as an index, the coumarin was detected at 268 nm by UV. Results The absorbency and concentration of phellopterin had a good linear relationship in the range of 2.65-7.94 ug/mL,R2=0.999. The average recovery rate was 103.84 % with RSD of 1.75 % （n=6）. Conclusion The method of content determination is simple and repeatable, it has laid a foundation for the quality evaluation on Csmyrnioides.

关 键 词：明党参 总香豆素 紫外分光光度法 含量测定 

分 类 号：R927.2[医药卫生—药学]