雏鹅冷应激反应中HPT轴HSP70mRNA的动态表达规律  

HPT Axis HSP70 mRNA Dynamic Expression During Cold Stress of Goose

作　　者：屠云洁[1,2] 陈国宏[2] 耿照玉[3] 苏一军[1] 王克华[1] 

机构地区：[1]中国农业科学院家禽研究所,江苏扬州225003 [2]扬州大学动物科学与技术学院,江苏扬州225009 [3]安徽农业大学动物科技学院,合肥230036

出　　处：《中国农业科学》2010年第7期1473-1479,共7页Scientia Agricultura Sinica

基　　金：国家科技支撑计划项目(2008BADC1B03;2008BADC1B01)

摘　　要：【目的】研究急性冷应激处理时,HSP70基因mRNA在下丘脑、垂体和甲状腺(HPT轴)中的表达规律。【方法】以7日龄皖西白鹅为研究对象,利用实时荧光定量逆转录PCR技术,分析(12±1)℃室温条件下雏鹅HSP70 mRNA在HPT轴中的动态表达规律。【结果】下丘脑HSP70 mRNA转录量在冷应激1.5、6和9h显著升高,其它时间点均恢复到正常水平;在应激结束后6h内HSP70 mRNA转录水平均显著升高,应激结束后6h转录水平最高,随后又迅速下降到室温下的转录水平。垂体HSP70 mRNA转录量在冷应激0.5、1.5、6和12h时显著降低,其它应激时间均升高到应激前的水平,应激结束后0.5、3和6h转录水平也显著低于应激前的水平,随后逐渐升高到应激前的正常水平。甲状腺HSP70 mRNA转录量在冷应激0.5h时显著升高,随后迅速下降到应激前的水平,应激3、6和12h时显著降低。应激结束后0.5h和3h转录量显著升高,其它时间点逐渐恢复到正常水平。【结论】雏鹅冷应激反应中,HSP70基因在下丘脑、垂体、甲状腺(HPT轴)中的表达规律是不同的,在不同的组织中有不同的调控规律。下丘脑HSP70 mRNA转录水平最高,表达量总体呈现明显上升趋势,是冷应激反应时的正调控基因;而它在垂体和甲状腺中的表达总体是被抑制的,是垂体和甲状腺冷应激反应时的负调控基因。[ Objective ] The objective of this study was to analyze the expression of HSP70 mRNA in Hypothalamic-Pituitary-Thyroid （HPT） axis under cold stress （12±1℃）. [Method] The dynamic expression of HSP70 mRNA in HPT axis of 7 d Wanxi White goose was got utilizing real-time fluorescent quantification reverse transcriptase PCR （FQ-RT-PCR）. [ Result ] Hypothalamic HSP70 mRNA transcription level went up significantly at1.5, 6, and 9 h of cold stress; It decreased to normal level in the other times. It increased again after cold exposure for 6 hours, moreover, it went up to the highest level at 6 h. Hereafter it declined to normal level. Pituitary HSP70 mRNA transcription level descended significantly at 0.5, 1.5, 6, and 12 h . It went up again gradually to normal level in the next 12 hours. It decreased again significantly at 0.5, 3, 6 h after cold exposure, hereafter gradually increased to normal level. Thyroid HSP70 mRNA transcription level went up quickly at 0.5h, hereafter decreased at 3, 6, 12 h significantly under cold stress. It went up significantly at 0.5h and 3h and it was down-regulated gradually to normal level in the other times after cold stress. [Conclusion] The expression rule of HSP70 mRNA was different in Hypothalamic-Pituitary-Thyroid （HPT） axis. Hypothalamic HSP70 mRNA transcription level was the highest which showed going up trend as a whole. HSP70 was the up-regulation gene of hypothalamic in cold stress reaction. Pituitary and thyroid HSP70 mRNA transcription level was repressed which showed that it was the down-regulation gene of pituitary and thyroid in cold stress reaction.

关 键 词：冷应激 HSP70 表达 荧光定量PCR 鹅 

分 类 号：S858.31[农业科学—临床兽医学]