两株植物内生巨大芽孢杆菌的生物学特性比较  

Comparative Analysis on the Biologic Characters of Two Endophytic Bacillus megaterium

作　　者：杨胜远[1] 韦锦[2] 李云[1] 

机构地区：[1]韩山师范学院生物系,潮州521041 [2]韩山师范学院图书馆,潮州521041

出　　处：《生物技术通报》2010年第3期185-190,共6页Biotechnology Bulletin

基　　金：广东省自然科学基金项目(8452104101001546);国家星火计划项目(2008GA780032);韩山师范学院科研基金项目

摘　　要：分别从爬山虎和金银花的茎中分离和鉴定获得了两株植物内生巨大芽孢杆菌(Bacillus megaterium),并对其形态特征、生理生化特征、培养及生理特征进行研究和比较。结果显示,在菌体的大小、耐盐能力、利用柠檬酸盐和在10°C下生长情况、D-木糖的代谢和产卵黄磷脂酶情况等方面,不同植物的内生巨大芽孢杆菌间以及植物内生菌与外源菌间都存在较大差异。结果表明,表型分类法在植物内生菌的鉴定中存在一定局限。Two endophytic bacteria,including Bacillus megaterium EJC-1 and B. megaterium EJH-7,were isolated from the stems of Parthenocissus tricuspidata （ Sieb. et Zucc） Planch and Lonicera japonica Thunb, respectively. The morphologic characters, culture characters, physiological and biochemical characters of the two strains were also investigated. The results indicated that some key biolog- ic characters,such as cells sizes, salt tolerance, citrate utilization, the ability of growth at 10 C,D-xylose metabolism and lecithinase pro- ducing,were different between the endophytic B. megaterium EJC-1 and B. megaterium EJH-7,and between the endophytic and non-endophytic B. megaterium. The results implied the phenotypie taxonomy was not sufficient for the application of the taxonomy on endophytic bacteria.

关 键 词：爬山虎 金银花 植物内生菌 巨大芽孢杆菌 生物学特性 

分 类 号：Q938[生物学—微生物学]