捷安肽素高产突变株96孔板筛选方法的建立  

Establishnent of a 96-well Microplate Screening Method for High Yield Strains of Jiean-peptide

作　　者：陈芬[1,2] 张晓勇[1] 钟娟[1] 周金燕[1] 李志东[1] 杨杰[1] 谭红[1] 

机构地区：[1]中国科学院成都生物研究所应用与环境微生物研究中心,成都610041 [2]中国科学院研究生院生命科学院,北京100049

出　　处：《生物技术通报》2010年第3期203-206,共4页Biotechnology Bulletin

基　　金：中国科学院知识创新工程重要方向项目(KSCX2-YW-G-039-2)

摘　　要：建立了一种快速灵敏地筛选出捷安肽素(JAA)高产突变株的方法。主要利用96多孔板固体培养菌体,用50%乙醇为最佳浸提液浸提4h,滤纸片法检测JAA生物活性的点样量为100μL,筛选出5株高产突变株,经摇瓶复筛选出2株高产突变株,经一剂量法检测,其抑菌圈直径较出发菌株提高了14%左右。A more rapid and sensitive screening method was constructed in this article in order to screen out the high-yielding mutants of Jiean-peptide（JAA）. The method included solid culture strains in 96-well mieroplate, extraction active ingredients of JAA with 50% ethanol for four hours,the filter paper method to detect biological activity of JAA. 100 p.L was the most appropriate testing dosage for the filter paper method. Five high-yield mutants were obtained by the screening method. Two of them were chosen, through shakeflask fermentation,which ensured they were high-producing strains. Diameters of antiblastic circles of them were increased by about 14% compared with the starting strain through one dosage technique.

关 键 词：捷安肽素 96孔板 浸提 滤纸片法 初筛方法 

分 类 号：S482.2[农业科学—农药学]