银杏黄酮苷元对氧化低密度脂蛋白所致内皮细胞功能的影响  

Effects of ginkgo aglycone on ox-LDL induced endothelial dysfunction

作　　者：何艳[1] 付永昕[1] 吴立荣[1] 方颖[1] 李屏[1] 李安敏[1] 

机构地区：[1]贵州省贵阳医学院附属医院心内科,贵阳550005

出　　处：《重庆医学》2010年第6期645-647,共3页Chongqing medicine

基　　金：贵州省优秀科技教育人才省长专项资金项目[黔省专合字(2005)225号]

摘　　要：目的探讨银杏黄酮苷元(GA)对氧化低密度脂蛋白(ox-LDL)诱导脐静脉内皮细胞株ECV304单核细胞趋化蛋白-1(MCP-1)和植物血凝素样低密度脂蛋白受体-1(LOX-1)表达的调节作用。方法应用逆转录-聚合酶链式反应(RT-PCR)、酶联免疫吸附反应(ELISA)、SP免疫组化法等,探讨ox-LDL诱导下内皮细胞表达MCP-1、LOX-1及银杏黄酮苷元的干预作用。结果6.25～25 mg/L银杏黄酮苷元与脐静脉内皮细胞株ECV304共同培养6～48 h可显著抑制ox-LDL诱导的内皮细胞MCP-1、LOX-1 mRNA和蛋白表达(P<0.05),具有浓度、时间效应关系(P<0.05)。LOX-1拮抗剂PIA可抑制ox-LDL诱导的内皮细胞LOX-1、MCP-1 mRNA和蛋白表达(P<0.05)。结论银杏黄酮苷元可显著抑制ox-LDL诱导的内皮细胞黏附因子MCP-1表达,该作用可能通过抑制LOX-1的表达来实现。Objective To investigate the effects of ginkgo aglycone（GA） on ox-LDL induced gene and protein expression of monocyte chemoattractant protein-1 （MCP-1） and lectinqike oxidized low density lipoprotein receptor-1 （LOXq） in human umbilical vein endothelial cells 304. Methods RT-PCR, ELISA assay, SP immunohistochemistry assay was used to detect the effect of ox- LDL and GA on expression of MCP 1, LOX-1 in endothelial cells. Results 6. 25--25 mg/L GA could inhibit the expression of MCP-1 and LOX-1 induced by ox LDL significantly（P〈0.05）,and to some extend,its effect appeared dependent on the drug con- centration and its action time（P〈0.05）. LOX-1 receptor inhibitor could inhibit the expression of LOX 1 and MCP-1 induced by ox- LDI.（P〈0.05）. Conclusion GA could inhibit significantly the expression of MCP-1 induced by ox-LDI.,while GA might imple- ment its anti-adhesive function by inhibiting expression of LOX-1.

关 键 词：:银杏黄酮苷元 氧化低密度脂蛋白 单核细胞趋化蛋白-1 植物血凝素样低密度脂蛋白受体-1 

分 类 号：R285.5[医药卫生—中药学]