超声波对恶臭假单孢菌生物转化和枯草芽孢杆菌原生质体形成的影响  

Study on Biologic Conversion of Pseudomonas Putida and Protoplast Formation of Bacillus Subtilis by Using Ultrasonic Wave

作　　者：冯瑞山[1] 徐秀兰[1] 李江宁[2] 奚涛[1] 袁忆丰[3] 

机构地区：[1]中国药科大学生物制药学院,南京210009 [2]中国药科大学 [3]南京大学声学研究所近代声学国家重点实验室,南京210093

出　　处：《药物生物技术》1998年第4期229-232,共4页Pharmaceutical Biotechnology

摘　　要：为探索超声波对恶臭假单孢菌生物转化和枯草芽孢杆菌原生质体形成的影响 ,用频率为 50 0 k Hz,声功率为 1 0 W/ cm2 低强度超声波辐照恶臭假单孢菌 ,其海因酶转化底物效率提高了60 %。枯草芽孢杆菌在溶菌酶作用中 ,同时伴随超声辐照 ,其原生质体形成速率明显加快 ,提前 30～ 60 min。Study on biologic conversion of Pseudomonas putida and protoplast formation of Bacillas subtilis by using ultrasonic wave. The culture of Pseudomonas putide was irratiated by ultrasonic wave with 500kHz 10W/cm 2, the percentange of biological conversion increased by 60%. When the culture of Bacillus subtilis was treated by lysozyme and irratiated by ultrasonic wave at the same time, Protoplast formation was accelerated clearly, and the rate of regeneration was increased.

关 键 词：海因酶 生物转化 原生质体 超声波 恶臭假单孢菌 

分 类 号：Q681[生物学—生物物理学] Q939.123