亮叶忍冬外植体灭菌方法的研究  

Study on the Sterilization Materials of Lonicera nitida'Maigrun'

作　　者：王丹[1] 李洪娜[1] 骆建霞[1] 柴慈江[1] 

机构地区：[1]天津农学院园艺系,天津300384

出　　处：《北方园艺》2010年第6期150-152,共3页Northern Horticulture

摘　　要：以亮叶忍冬带芽茎段为试材,对影响外植体组织培养的灭菌方法进行研究。结果表明:先用70%酒精进行表面浸润消毒处理15 s,然后用2%次氯酸钠处理15 min,外植体污染率低,存活率高,发芽率高,新梢生长量也相对较高;在接种之前保留部分外植体叶片可以降低污染率,促进发芽及新梢生长;培养基中添加蔗糖可以促进发芽。The effects of investigated by using Lonicera nitida ‘Maigrun’ as testing material. The results indicated that the most sterilized materials could be obtained,, and the explants with lower contaminated rate,higher survival rate,relatively higher germination rate and longer shoot growth were observed when the explant treated with 70% alcohol as surface sterilization for 15 rain, then with 2% NaClO for 15 min. Contaminated rate could be declined, while germination and shoot growth were enhanced by keeping explant with parts of leaves before inoculation. Increasing cane sugar in the medium could promote the germination effectively.

关 键 词：亮叶忍冬 外植体 灭菌方法 污染率 发芽率 

分 类 号：S793.9[农业科学—林木遗传育种]