陈化期间不同地区烤烟叶片中酶活性变化研究  

A Study on the Enzymatic Activity in Flue-cured Tobacco Leaf in Different Areas during the Aging Stage

作　　者：赵铭钦[1] 王付锋[1] 张志逢[1] 陈秋会[2] 

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]浙江大学环境与资源学院,浙江杭州310029

出　　处：《江西农业大学学报》2010年第1期15-19,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：吉林烟草工业有限责任公司重大科技攻关资助项目(JY2006012)

摘　　要：探索在河南气候条件下陈化的不同地区烤烟中多酚氧化酶(PPO)、过氧化物酶(POD)、脂氧合酶(LOX)、苯丙氨酸解氨酶(PAL)活性的变化规律。结果表明:(1)在不同陈化阶段,国内4个烟区烟叶中各酶活性表现为在烟叶陈化初期呈现逐渐增加的趋势,到陈化6个月或者9个月时酶活性达到最大值,之后酶活性逐渐降低。(2)在24个月的陈化进程中,各地区的烟叶中4种酶的酶活均较其陈化初始降幅有所不同,其中湖南烟区烤烟过氧化物酶和苯丙氨酸解氨酶活性降低幅度最大,分别约为77.01%和14.15%;多酚氧化酶则以云南烟叶降幅最大,为66.67%;贵州烟叶的脂氧合酶活性降低最多,为68.93%。(3)津巴布韦烟区烤烟各酶活在15～39个月时逐渐降低,且活性相对较低,以脂氧合酶活性降幅最大。In order to explore the changing laws of the activity of the polypgenol oxidase,peroxidase,lipoxygenase and phenylalanine ammonialyase in the flue-cured tobacco leaves during the aging stage in different areas under the Henan atmosphere condition.The results showed that：（1） at different aging times,the activity of every enzyme gradually increased in the beginning period,and the amount peaked at the aging of the 6th or 9th months,then diminished gradually;（2） the activities of enzymes in different areas had different decreading amplitudes during the 24 months course of aging,the decreading amplitudes of the activity of POD and PAL were the largest in Hunan area,they decreased by 77.01% and 4.15% respectively;the activity of PPO in Yunnan area had the largest decreading amplitude,and reached 66.67% ;the activity of LOX in the flue-crued tobacco leaves in Guizhou had the largest decreading amplitude（68.93%）;（3）the activity of every enzyme in the flue-cured tobacco leaves in Zimbabwe decreased gradually during the 15th stage from the to the 39th months and the activity was comparatively lower,especilly the activity of LOX had the largest decreading amplitude.

关 键 词：烤烟 陈化 酶活性 

分 类 号：S572.01[农业科学—烟草工业]