萜类蚜虫拒食剂的筛选研究  

Screening of Aphid Antifeedant from Terpenoid Compounds

作　　者：韩招久[1] 郑卫青[1,2] 姜志宽[1] 钱万红[1] 王宗德[2] 陈金珠[2] 

机构地区：[1]南京军区军事医学研究所,江苏南京210002 [2]江西农业大学,江西南昌330045

出　　处：《江西农业大学学报》2010年第1期78-84,共7页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江苏省自然科学基金资助项目(BK2005003);江西省自然科学基金资助项目(2007GZN0262);江西省教育厅项目(GJJ08182);江西省青年科学家(井冈之星)培养对象计划资助项目

摘　　要：萜类化合物是重要的昆虫拒食剂类型。研究采用叶碟浸液法测试松节油、羟基香茅醛、薄荷醇及其衍生合成的多个系列的萜类化合物对蔬菜害虫萝卜蚜(Lipaphis erysimi)的拒食活性。结果显示,当质量浓度为10mg/mL时,24h后有22种化合物的拒食率高于75%,其中诺卜醇、内型异莰烷基甲醇乙酸酯、4-(1-甲基乙烯基)-1-环己烯-1-乙醇丙酸酯、诺卜甲基醚、8-羟基别二氢葛缕醇、薄荷醇、8-羟基别二氢葛缕醇甲酸酯、8-羟基别二氢葛缕醇乙酸酯、8-羟基别二氢葛缕醇丙酸酯、羟基香茅醛乙基醚、蒎酮酸甲酯等11种化合物的拒食率达到100%。当浓度为1mg/mL时,这些化合物的拒食活性出现了较大的差别,其中2个化合物即内型异莰烷基甲醇丙酸酯和内型1-异莰烷基-3-己醇乙酸酯的拒食活性最高,且持效性较好。系列等比浓度测试结果显示,内型1-异莰烷基-3-己醇乙酸酯的剂量-活性回归方程的坡度值在12,24,36,48,72h为0.47～1.72,拒食中浓度(AFC50)为4.0～12.1mg/mL。8号化合物(内型异莰烷基甲醇丙酸酯)的坡度值为1.08～2.91,AFC50为2.6～7.0mg/mL,表现出良好的正向剂量活性效应和持效性,是一种具有应用前景的蚜虫拒食剂。Terpenoid compounds are important chemicals in insect antifeedants.In this study,the antefeedant activities of turpentine oil,hydroxycitronellal,menthol and their derivatives synthesized in a project against turnip aphid,Lipaphis erysimi（Kaltenbach）,were tested using the method of dipped leaf discs.22 compounds showed antifeedant activities more than 75% 24 h after the treatment at the concentration of 10 mg/mL,and eleven of which,i.e.nopol,endo-isocamphanyl-methyl acetate,4-（1-methylethylene）-1-cyclohexenyl-1-ethol propionate,nopyl methyl ether,8-hydroxylcarveol,menthol,8-hydroxylcarveol formate,8-hydroxylcarveol acetate,8-hydroxylcarveol propionate,hydroxycitronellal ethyl ether,methyl pinonate,held the antifeedant rates as much as 100%.15 compounds were further tested with the concentration of 1 mg/mL,and their activities were differentiated much with the antefeedant rates from-36% to 79% 24 h later.Compounds 8 and 10,namely endo-isocamphanyl-methyl propionate and endo-1-isocamphanyl-3-hexyl acetate,comprehensively held the best action and residual efficacy.The results of probit analysisshowed that the slopes of dosage-action linear regression of compound 8 varied from 1.08 to 2.91;AFC50s were from 2.6 mg/mL to 7.0 mg/mL at the observation time points of 12 h,24 h,36 h,48 h and 72 h after treatment.The slopes of compound 10 varied from 0.47 to 1.72;AFC50 s were from 4.6 mg/mL to 12.2 mg/mL.It is suggested that endo-isocamphanyl-methyl propionate would be a promising antifeedant in aphis control.

关 键 词：萜类化合物 萝卜蚜 拒食活性 

分 类 号：Q541[生物学—生物化学] S481.1[农业科学—农药学]