白羽番鸭脂联素基因外显子1与肉质、TC和TG的关联分析  

Polymorphism in Exon-1 of Adiponectin Gene and Its Association with Meat Quality and TC and TG in White Muscovy

作　　者：张依裕[1,2] 徐琪[1] 段修军 张海波[1] 赵文明[1] 孙国波 陈国宏[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]贵州大学动物科学学院,贵州贵阳550025 [3]江苏省畜牧兽医职业技术学院,江苏泰州225300

出　　处：《江西农业大学学报》2010年第1期115-118,134,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家科技支撑计划重大专项(2006BDA01A09);国家科技支撑计划重点项目(2006BDA14B06);现代农业产业技术体系建设专项资金(nycytx-45-04)联合资助

摘　　要：采用PCR-SSCP法对白羽番鸭脂联素基因外显子1进行SNP检测,并分析SNP对肉质、血清总胆固醇(TC)和甘油三酯(TG)的遗传效应。结果表明,检测到3种基因型(AA、AB和BB)和2个等位基因(A和B),AA和A分别为优势基因型和优势等位基因,序列比对发现在外显子1存在1个SNP位点:A167G,为沉默突变,该位点在白羽番鸭群体中表现为中度多态,处于Hardy-Weinberg平衡状态(P>0.05);最小二乘法分析表明,AA基因型个体的肌内脂肪(IMF)和血清总胆固醇(TC)显著高于BB基因型个体(P<0.05),而失水率则显著低于BB基因型个体(P<0.05)。结果揭示,A167G位点对脂肪沉积、失水率和胆固醇浓度可能有显著的遗传效应。The 230 bp fragment of adiponectin gene exon 1 of White Muscovy was amplified with the primer designed from Cairina moschata DNA sequence（GQ873067）,and its polymorphism was detected by PCR-SSCP method,and then its association with meat quality and serum total cholesterol（TC） and triglyceride（TG） were analyzed.2 alleles（A and B） and 3 genotypes（AA,AB and BB） were observed,in which A and AA were predominant.One SNP（A167G） was found in exon 1 of adiponectin gene,which belonged to silent mutation.The A167G loci belonged to medium polymorphism,and it was in accordance with Hardy-Weinberg equilibrium（P〉0.05）.The birds of genotype AA had significantly higher IMF and TC than those of genotype BB（P〈0.05）.The birds of genotype AA had significantly lower percentage of chest muscle loss water than those of genotype BB（P〈0.05）.The study suggested that the A167G loci has significant genetic effect on fatty deposition,percentage of chest muscle loss water and TC concentration.

关 键 词：白羽番鸭 脂联素基因 PCR-SSCP 关联分析 

分 类 号：S834[农业科学—畜牧学]