不同pH值条件下的丝氨酸标准曲线  

Serine standard curves at different pH

作　　者：彭继明[1] 张佑红[1] 徐鹏[1] 陈林[1] 马静[1] 危威[1] 

机构地区：[1]武汉工程大学化工与制药学院绿色化工过程省部共建教育部重点实验室,湖北武汉430074

出　　处：《武汉工程大学学报》2010年第3期20-24,共5页Journal of Wuhan Institute of Technology

摘　　要：根据丝氨酸与茚三酮反应后溶液在570nm处有最大吸光值，用分光光度法测定丝氨酸含量，确定了丝氨酸与茚三酮反应的最佳时间和相对用量．为了准确测定磷脂制备体系中丝氨酸含量，绘制了pH值分别为4．5，5．0，和6．0时的丝氨酸标准曲线．结果表明：pH值为4．5时，丝氨酸的含量与A570值在0～0．20g／L范围内呈良好的线性关系y=5．595x＋0．056（R^2=0．9912）；pH值为5．0时丝氨酸的含量与A570值在0～0．10g／L范围内呈良好的线性关系y=10．59x＋0．023（R^2=0．9985）；pH值为6．0时，丝氨酸的含量与A570值在0～0．04g／L范围内呈良好的线性关系y=37．388x＋0．0052（R^2=0．9915）．According to the principle of color reaction between α-amino-acids and ninhydrin, ninhydrin spectrophotometric method of determining the serine level has been set up and A570 value was determined. The optimal experimental conditions of ninhydrin-spectrophotometry such as pH, the relative amount of ninhydrin added and reaction time have been investigated. The results showed that： the relationship of the amount of serine in 0--0.20 g/L with A570 at pH of 4.5 was a good linear y= 5.595x-l-0.056（R2=0. 991 2）; For the serine in 0--0. 10 g/L at pH of 5.0, the linear correlation equation is y=10.59x＋0. 023（Rz =0. 998 5） ; For the serine in 0--0.04 g/L with A570 at pH of 6.0, the linear correlation equation is y=37. 388x＋0. 005 2（R2 =0. 991 5）.

关 键 词：分光光度法 L-丝氨酸 线性关系 标准曲线 

分 类 号：Q517[生物学—生物化学]