人骨髓间充质干细胞体外诱导分化为心肌样细胞的L型钙通道研究  

The L-type Ca^(2+) channels of the cardiomyocyte-like cells induced to differentiate from human marrow mesenchymal stem cells in vitro.

作　　者：邓方阁[1] 李玉林[2] 

机构地区：[1]广州医学院第一附属医院广州呼吸病研究所呼吸疾病国家重点实验室,510120 [2]吉林大学白求恩医学院病理生物学教育部国家重点实验室,130021

出　　处：《国际心血管病杂志》2010年第2期116-118,共3页International Journal of Cardiovascular Disease

基　　金：中国博士后基金(20090450853)

摘　　要：目的:观察人骨髓间充质干细胞(hMSCs)诱导分化为心肌样细胞的电生理特征,L型钙离子通道的电流强度。方法:体外分离培养hMSCs,以5-氮胞苷诱导分化为心肌样细胞。分别以体外分离培养的原代搏动的SD乳鼠心肌细胞(CMs)和同代次的hMSCs为阳性对照和阴性对照,利用膜片钳技术观察诱导分化为心肌样细胞的电生理特征L型钙离子通道的电流强度。结果:在20个心肌样细胞中,有6个细胞可记录到对硝苯地平敏感的内向钙电流,其最大内向峰值电流(ICa-Peak)在0mV为(112.60±8.26)pA;而hMSCs和乳鼠的CMs的ICa-Peak分别为(96.67±13.50)pA和(263.86±39.01)pA(n=6),将诱导的心肌样细胞与未经诱导的hMSCs的ICa-Peak进行比较,P<0.05具有统计学意义。结论:hMSCs经5-氮胞苷诱导后,其L型钙离子通道的钙电流增加,在电生理特性上具有向CMs分化的潜能。Objective：atch clamp technique was utilized to investigate electrophysiological characteristics on electric current intensity of L-type Ca^2＋ channels of the cardiomyocyte-like cells induced to differentiate from human marrow mesenchymal stem cells （hMSCs） in vitro. Methods： Isolated and cultured hMSCs were differenti-ated into cardiomyocyte-like cells by 5-azacytine in vitro. Same generation of hMSCs with cardiomyocyte-like cells and cultured primary beating SD suckling rats＂ cardiomyocytes （CMs） served as negative and positive control groups, hMSCs, cardiomyocyte-like cells and CMs were examined for their electrophysiological characteristics on electric current intensity of L-type Ca2. channels by patch clamp technique. Results：The patch clamp examination showed that there were 6 out of 20 cardiomyocyte-like cells recorded inward calcium current which was sensitive to nifedipine. The peak inward calcium current （Ica-Peak） was （ 112.60 ± 8, 26 ） pA at 0mV, whereas the Ica-Peak of hM-SCs and CMs were （ 96. 67 ± 13.50 ） pA and （ 263.86 ± 39. 01 ） pA, respectively. There was significant difference between the ICa-Peak of cardiomyocyte-like cells induced from hMSCs and that of uninduced-hMSCs, with a P〈0.05. Conclusion：The calcium current of L-type Ca^2＋ channels increases in hMSCs induced by 5-azacytine, which predisposes hMSCs to differentiate into cardiomyocyte in the electrophysiological characteristics.

关 键 词：人骨髓间充质干细胞 心肌样细胞 膜片钳 电生理 L型钙离子通道 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]