催化动力学光度法测定接骨木中槲皮素  

Determination of Quercetin in Sambucus by Catalytic Kinetic Spectrophotometry

作　　者：高扬[1] 翁慧[1] 刘雅静[1] 李英杰[1] 马莹莹[1] 张力[1] 包玉敏[1] 

机构地区：[1]内蒙古民族大学化学化工学院,内蒙古通辽028043

出　　处：《内蒙古民族大学学报（自然科学版）》2010年第1期24-25,共2页Journal of Inner Mongolia Minzu University：Natural Sciences

基　　金：内蒙古自治区自然科学基金资助项目(200607010209);内蒙古高等学校自然科学基金资助项目(NJZY07138)

摘　　要：基于稀H2SO4介质中,槲皮素对KBrO3氧化甲基橙褪色反应的明显催化作用,建立了测定槲皮素的动力学光度法.采用超声波法和回流法提取接骨木中槲皮素,用催化动力学光度法测定其含量,检测波长为517 nm,测定槲皮素的线性范围为0.00～0.05 mg/L,检出限为0.002 mg/L.两种提取方法测得接骨木中槲皮素含量分别为2.522 mg/g和3.420 mg/g,平均回收率分别为91.3%和96.9%.结果表明,超声波辅助萃取法简便快捷,提取率高.A catalytic kinetic spectrophotometric method for the determination of quercetin was proposed.The method was based on a discoloring reaction of methyl red by quercetin as catalyst in the dilute sulfuric aeid.Results：A linear limit of 0.00mg/L-0.05mg/L and a mean recovery of 91.3% and 96.9% were obtained.It is concluded that this method is simple and rapid.

关 键 词：接骨木 槲皮素 甲基红 动力学光度法 

分 类 号：O657.32[理学—分析化学]