B组柯萨奇病毒对心肌细胞感染模式的研究  

A study of the effect of infection modes of Coxsackie virus B on cardiomyocytes

作　　者：张中海[1] 张琦[2] 闫长栋[1] 钟照华[3] 

机构地区：[1]徐州医学院生理学教研室,江苏徐州221004 [2]徐州医学院实验动物中心 [3]哈尔滨医科大学微生物学教研室,黑龙江哈尔滨150086

出　　处：《徐州医学院学报》2010年第3期155-160,共6页Acta Academiae Medicinae Xuzhou

基　　金：徐州医学院院课题(08KJ18)

摘　　要：目的观察B组柯萨奇病毒(Coxsackie virus B,CVB)在原代心肌细胞中的感染特点,从而研究CVB对心肌细胞的致病机制。方法原代培养Balb/c乳鼠心肌细胞,并获得纯化的心肌细胞。使用B组柯萨奇病毒嗜心肌毒株3型(CVB3m)攻击原代心肌细胞,观察病毒感染心肌细胞后心肌细胞的细胞病变、超微结构和心肌酶的变化。结果成功原代培养了Balb/c乳鼠心肌细胞。心肌细胞在病毒感染后,细胞病变(CPE)不明显,与在HeLa等细胞中的典型CPE不同。心肌细胞在CVB3m感染后保持细胞形态和博动超过2周时间。电镜显示心肌细胞结构正常,细胞器数量增加,在心肌细胞胞质中可见晶格样病毒颗粒排列。培养细胞的上清液中心肌酶升高。结论CVB在原代心肌细胞中表现为持续感染,CVB在心肌细胞中的致病机制不同于HeLa细胞。Objective To investigate the characteristics of Coxsackie virus B （CVB） on primarily cultured myocardial ceils and to explore the pathogenesis of cardiomyocytes. Methods Cardiomyocytes from Balb/c suckling mouse were cultured in vitro, and purified myocardial cells were obtained. The cultured myocardial cells were attacked with CVB3m, a myocardial tropic Coxsackie virus B strain. The cytopathic effect （CPE） and uhrastructure of the CVB3m - infected myocardial cells were observed. The variations of myocardial enzymes in the supernant of the culture medium were determined. Results The cardiomyocytes from neonate Balb/c mice were successfully cultured. No apparent CPE could be observed in the cultured myocardial cells following the CVB3m attack, which was different from the typical CPE in the infections of HeLa cells. The cardiomyocytes retained intact morphology and pulsation for over two weeks as of the infection. The electronic microscopy showed that the myocardial cells had normal ultrastructure, with an increase in the number of organelles and visible crystally - arranged viral particles in the cytoplasm. The level of myocardial enzymes in the supernatant of the infected myocardial cells was higher than that of the control group. Conclusion CVB can persistently infect cardiomyocytes. The pathogenesis of CVB on myocardial cells differs from that in HeLa cell.

关 键 词：心肌细胞 B组柯萨奇病毒 Balb/c乳鼠 原代细胞培养 

分 类 号：R512.5[医药卫生—内科学]