ICOSL单克隆抗体对BXSB狼疮鼠病变的影响  

Blocking ICOS co-stimulation suppresses lupus-like syndrome in autoimmune BXSB mice

作　　者：丁涵露[1] 吴雄飞[2] 

机构地区：[1]四川省人民医院肾内科,四川成都610072 [2]第三军医大学西南医院肾科,重庆400038

出　　处：《四川医学》2010年第3期285-287,共3页Sichuan Medical Journal

摘　　要：目的观察大鼠抗小鼠ICOSL多克隆抗体抑制ICOS(可诱导共刺激分子)信号通路对BXSB狼疮鼠病变的影响。方法大鼠抗小鼠ICOSL(可诱导共刺激分子配体)抗体尾静脉注射BXSB狼疮鼠,100μg/次,3次/周,共4周,对照组以相同量PBS注射,在治疗后观察血IgG型dsDNA抗体、尿蛋白、肾小球IgG沉积以及肾组织病理变化。结果实验组狼疮鼠的蛋白尿和IgG型dsDNA抗体较对照组明显减少(P<0.01),IgG在肾组织沉积以及肾组织病理损伤也较对照组减轻(P<0.01)。结论抑制ICOS信号通路可减轻BXSB狼疮鼠病变。Objective Study the role of blocking ICOS positive co-stimulation pathways in preventing the onset of murine BXSB lupus.Methods We developed anti-ICOSL（B7h）mAb to block ICOS-mediated co-stimulation to prevent lupus nephritis in BXSB mice.Eight weeks old BXSB male mice were randomly divided into two groups and treated with PBS and 100 μg doses of ICOSL antibody three times a week for four weeks.All the above reagents were administrated via tail vein.Blood samples were collected from the tail veins of treated BXSB mice to examine proteinuria and IgG type anti-double stranded DNA antibody in serum.We also examine the severity of nephritis and IgG deposition in glomerular.Results The therapy dramatically delayed the onset of proteinuria,effectively inhibited IgG autoantibody production,and significantly reduced hypercellularity and deposition of IgG in glomeruli compared with controls（P〈0.01）.Conclusion Our results indicate the therapeutic potential of blockade of ICOS-B7h co-stimulation by ICOSL antibody in the prevention of human lupus nephritis.

关 键 词：可诱导共刺激分子 可诱导共刺激分子配体 狼疮性肾炎 BXSB狼疮鼠 

分 类 号：R593.242[医药卫生—内科学]