小麦DREB转录因子TaAIDFa的互作蛋白筛选  

Screening of the Interacting Proteins of DREB Transcription Factor TaAIDFa in Wheat

作　　者：郑甲成[1,2] 徐兆师[2] 胡银岗[1] 刘婷[1] 李连城[2] 陈明[2] 马有志[1,2] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]国家农作物基因资源与基因改良重大科学工程,农业部作物遗传育种重点开放实验室,中国农业科学院作物科学研究所,北京100081

出　　处：《麦类作物学报》2010年第2期189-193,共5页Journal of Triticeae Crops

基　　金：国家高技术研究发展计划(863计划)项目(2007AA10Z130);国家自然科学基金项目(30700504);转基因生物新品种培育科技重大专项(2009ZX08009-083B)

摘　　要：为进一步探讨DREB类蛋白的作用机理,以DREB类转录因子TaAIDFa为探针,利用酵母双杂交技术从cDNA文库中筛选TaAIDFa的互作蛋白,进而探讨DREB转录因子介导的互作网络,以初步阐明DREB转录因子的抗逆机制。结果表明,通过酵母双杂交从cDNA文库中筛选到84个克隆,对其进行测序和Blast序列比对分析,得到4类与TaAIDFa互作的候选蛋白。同源性分析发现,这4类候选蛋白多与信号转导或免疫过程有关,说明TaAIDFa参与了植物逆境胁迫下的信号转导,可调控与逆境胁迫相关基因的表达。DREB（dehydration responsive element binding protein） can combine specifically to DRE/CRT cis-acting elements,activate expression of stress-related genes,and improve the resistance of plants.In this research,TaAIDFa was used as a bait to screen interacting proteins by yeast two-hybrid system to explore the interacting network mediated by DREB and to illuminate the resistant mechanism.We abtained 84 clones which might interact with TaAIDFa.These clones were grouped into 4 types of candidate proteins according to their putative functions.The majority of four types of proteins related to signal transduction and immunologic process,which showed that TaAIDFa maybe invovled in the signal transduction under adversity,and played an essential role in regulating the expression of stress-resistance genes and improving plants resistance.

关 键 词：小麦 DREB 基因克隆 互作蛋白 

分 类 号：S512.1[农业科学—作物学] S332