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机构地区:[1]作物生物学国家重点实验室/山东农业大学生命科学学院,山东泰安271018 [2]浙江大学生命科学学院农业生态与工程研究所,浙江杭州310058
出 处:《山东农业科学》2010年第3期32-37,共6页Shandong Agricultural Sciences
基 金:国家自然科学基金重点项目(30730020);国家自然科学基金项目(30870405)资助
摘 要:采用盆栽受控试验法,设置无盐胁迫(0.046%)、轻度盐胁迫(0.2%)和重度盐胁迫(0.5%)3个盐分水平,研究了不同盐分下丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)对紫花苜蓿(Medicago sativaL.)生长量和耐盐性的影响。结果表明,随盐胁迫程度的增加,相同AMF处理的紫花苜蓿生长量显著降低(P<0.05),叶片游离脯氨酸、丙二醛含量和膜透性显著增加(P<0.05);与低AMF处理相比,3个盐分水平下高AMF处理的紫花苜蓿的生长量都显著增加(P<0.05),紫花苜蓿叶片游离脯氨酸、丙二醛含量和膜透性都显著降低(P<0.05),表明AMF能增加紫花苜蓿的耐盐性,缓解盐胁迫对紫花苜蓿生长的影响。A mesocosm experiment was conducted to test the effects of arbuscular mycorrhizal fungi (AMF) on the growth and salt tolerance of Medicago sativa L. under no salt stress (0.046%), slight salt stress (0.2%) and severe salt stress (0.5%). As the results, with the increasing of the salt stress degree, the growth significantly decreased, the free proline and malondialdehyde (MDA) contents and the membrane permeability in the leaves significantly increased with the same AMF treatment. Under the same salt stress, the growth significantly increased, the free proline and malondialdehyde (MDA) contents and the membrane permeability in the leaves significantly decreased with the higher AMF treatment than those with the lower AMF treatment. The results indicated that AMF could increase the salt tolerance of M. sativa and relieve the effect of salt stress on the growth of M. sativa.
关 键 词:盐胁迫 丛枝菌根真菌(AMF) 紫花苜蓿
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