用STR分型技术作亲权鉴定时判断标准的研究  

Strategy of Conclusion-Making for Paternity Testing with Short Tandem Repeats Genotyping

作　　者：伍新尧[1] 童大跃[1] 朱运良[1,2] 蔡贵庆[1] 陈勇[1] 孙宏钰[1] 

机构地区：[1]中山大学中山医学院法医学系,广东广州510080 [2]郑州大学基础医学院法医学教研室,河南郑州450001

出　　处：《中山大学学报（医学科学版）》2010年第1期1-6,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金："211工程"重点学科建设课题(4209008);广东省自然科学基金(97-128)

摘　　要：【目的】探讨STR分型技术作亲权鉴定时,判断是否存在亲生关系的标准。【方法】采用二项式分布定律P(m)=Cnm(1-π)n-mπm计算亲权鉴定时理论上应该检测的STR基因座数量,并用实际检案检验。【结果】确定以下的判断标准。三联体案:在累积PI值超过10000的前提下,①最少检测15个STR基因座(单个基因座非父排除率≥0.5714),若争议父子之间没有发现不符合遗传规律基因座(简称为矛盾基因座,下同);或②检测19个基因座只出现1个矛盾基因座;或③检测28个基因座,只发现2个矛盾基因座;或④检测35个或更多基因座,只发现3个矛盾基因座,作出"肯定亲生关系"的结论;若出现4个矛盾STR基因座,则作出"否定亲生关系"的结论。单亲案:在累计PI值≥10000前提下,①至少检测18个基因座(单个基因座非父排除率≥0.411),没有发现矛盾;或②检测29个以上,只发现1个矛盾基因座;或③检测41个或更多基因座,只有2个矛盾基因座,作出"不排除亲生关系"的结论;如果出现3个或3个以上矛盾STR基因座,则作出"否定亲生关系"的结论。本实验室实际检案表明合理。【结论】上述判断是否存在亲生关系标准符合科学、公正原则,值得推广应用。【Objective】 To propose a criterion for making conclusions on paternity tests based on STR genotyping.【Method】 To use binomial distribution formula to calculate minimal numbers of STR loci that must be tested for different scenarios in paternity testing.【Results】 We proposed a set of criteria for making STR paternity testing conclusions.For triplet tests,concluded ＂paternity positive ＂ for the following four cases when the cumulative paternity index （PI） was greater than 10 000 ： 1 ） no inconsistent STR locus was detected in 15 loci （PE 0.571 4 / locus） or 2） only one inconsistent STR locus was detected in 19 loci or 3） only two inconsistent STR loci were detected in 28 loci or 4） only three inconsistent STR loci were detected in 35 loci;otherwise,concluded ＂paternity negative＂ when at least four inconsistent STR loci had been detected.For single parent tests,concluded ＂paternity non-exclusive＂ for the following cases when the cumulative PI was greater than 10 000： 1） no inconsistent STR locus was detected in 18 loci （PE 0.411 / locus） or 2） only one inconsistent STR locus was detected in 29 loci or 3） two inconsistent STR loci were detected in 41 loci;concluded ＂paternity negative＂ when three or more inconsistent loci were detected.【Conclusion】 Our experience has proven that these criteria are robust in STR paternity testing.

关 键 词：短串联重复序列 亲权鉴定 父权指数(PI值) 标准 

分 类 号：Q987[生物学—遗传学]