白术茯苓汤不同配比水煎液中多糖的含量研究  

Different proportions of polysaccharide content in Baishu Fuling decoction with different amount of water

作　　者：彭腾[1] 贾波[1] 邱建平[1] 何宇新 彭成[1] 施翠瑛[1] 刘兴隆[1] 

机构地区：[1]成都中医药大学,成都610075 [2]西华大学生物工程学院,成都610039

出　　处：《药物分析杂志》2010年第3期393-395,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金资助项目(编号:30572296);成都中医药大学发展基金项目(编号:ZRYB200804)

摘　　要：目的:研究白术茯苓汤不同配比水煎液中多糖的含量;方法:水提醇沉法分离出粗多糖,采用蒽酮-硫酸法制得有色糖醛衍生物,用分光光度法进行含量测定。结果:加入0.3%的硫酸蒽酮溶液4mL,在水浴中加热10min,冷却10min时复方多糖含量测定条件最优。显示627nm处有最大吸收峰,线性关系良好(r=0.9997),平均回收率为97.3%。结论:白术单煎多糖提取的含量最高,茯苓所占比例越大,含量越低。该方法快速准确,重现性好。Objective：To study different proportions of polysaccharide content in Baishu Fuling decoction. Methods： The polysaccharide were extracted with hot water and precipitated with alcohol.The contents of polysaccharide were determined by sulfuric acid-anthrone method：by UV spectrophotometry.Result：The optimum determination conditions for polysaccbaride contentin in Baishu Fuling decoction were as follows：0.3% anthrone-sulfate solution 4 mL,heating for 10 min in water bath and cooling for 10 min.There is maximum absorption of 627 nm peak,good linear relationship（r=0.9997）,the average recovery was 97.3%.Conclusion：Atractylodes macrocephala single fry the highest content of polysaccharide extracted,the greater the proportion of Wdfiporia COCos,the lower content.The method is rapid,accurate,reproducible.

关 键 词：白术茯苓汤 配比 多糖含量 

分 类 号：R917[医药卫生—药物分析学]