箭叶淫羊藿提取液的高效液相色谱分离及淫羊藿苷的色谱制备  

HPLC separation of Epimedium sagittatum extract and chromatographic preparation of icariin

作　　者：常宗策[1,2] 成艳[3] 梁永民[1] 陈涛[2,4,5] 

机构地区：[1]兰州大学,兰州730000 [2]西安力邦制药有限公司,西安710075 [3]第四车医大学唐都医院传染科,西安710038 [4]陕西省脂质体工程技术研究中心,西安710075 [5]西北工业大学生命科学院,西安710072

出　　处：《药物分析杂志》2010年第3期415-419,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:分离制备淫羊藿苷,为箭叶淫羊藿及其制品的质量检测以及箭叶淫羊藿液相色谱指纹图谱的建立奠定了良好的分离分析基础。方法:分析Shim-pack VP ODS(150mm×4.6mm,5μm)柱,柱温:室温,流速:1.0mL·min-1;制备Shim-pack PREP C8(250mm×20mm,15μm,100),流速:5.0mL·min-1,检测波长:270nm,进样量:35mL,柱温室温。结果:反相色谱制备可以一步达到95%以上纯度,梯度分离能更好地将各组分分离。结论:反相高效液相色谱可用于制备箭叶淫羊藿中的淫羊藿苷;梯度分离可用于箭叶淫羊藿及其制品的质量检测。Objective：To prepare icariin with separation,and establish an appropriate separation analysis method for liquid chromatographic fingerprints of Epimedium sagittatum and its products.Method：The analysis was perfnmed with Shim-pack VP ODS（150 mm×4.6 mm,5 μm）at flow rate,column temperature at room temperature,1.0 mL·min-1.The preparation was performed with Shim-pack PREP C8（250 mm×20 mm,15 μm,100）at flow rate 5.0 mL·min-1,sample volume was 35 mL,detecting wavelength was set at 270 nm,and column temperature was room temperature.Results：The preparation of RP-HPLC could make the purity up to 95% with one-step,and the gradient separation also separates each component very well.Conclusion：By using the R P-HPLC,icariin was prepared successfully from Epimedium sagittatum.The gradient separation can be used for testing Epimedium sagittatum and its products.

关 键 词：箭叶淫羊藿 淫羊藿苷 反相液相色谱 制备分离 波谱分析 

分 类 号：R917[医药卫生—药物分析学]