毛细管气相色谱法同时测定苍术中茅术醇和β-桉叶醇含量  

Capillary gas chromatography simultaneous determination of hinesol and β-eudesmol in Rhizoma atractylodis

作　　者：吕美红[1] 严启新 梁慧 李勇 李姿 冯汉林 谢晓梅[1] 

机构地区：[1]安徽中医学院药学院,安徽省现代中药重点实验室,合肥230031 [2]深圳海王药业有限公司,深圳518057

出　　处：《药物分析杂志》2010年第3期460-464,共5页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立毛细管气相色谱法同时测定苍术中茅术醇与β-桉叶醇含量。方法:HP-INNOWax Polyethylene Glycol(30m×0.32mm×0.5μm)石英毛细管柱,氢火焰离子化检测器(FID),以正十五醇为内标物,气化室温度270℃,柱温185℃,氮气为载气,流速25mL.min-1。结果:茅术醇在0.102～0.819μg.mL-1范围内呈良好的线性关系(r=0.9999),β-桉叶醇在0.098～0.787μg.mL-1范围内呈良好的线性关系(r=0.9999);茅术醇平均回收率为100.9%(RSD=1.80%),β-桉叶醇平均回收率为99.6%(RSD=1.71%)。结论:本方法简便、准确、重现性好,可用于苍术药材及其饮片的质量控制。Objective：To establish gas chromatography method for simultaneous determination of the contents of hinesol and β-eudesmol in Rhizoma atractylodis.Methods：Chromatography conditions：1-Pentadecanol had been used as the internal standard substance in internal standard method;column：HP-INNOWax Polyethylene Glycol（30 m×0.32 mm×0.5 μm）;detector：Hydrogen flame ionization detector;injection chamber temperature：270 ℃;column temperature：185 ℃;carrier gas：N2, the flow rate was 25 mL·min-1.Results：The linear range of hinesol was 0.102-0.819 μg·mL-1（r=0.999 9）and the linear range ofβ-eudesmol was 0.0984-0.787 μg·mL-1（r=0.999 9）;The average recovery rate of hinesol was 100.9%（RSD=1.80%）,the average recovery rate of β-eudesmol was 99.6%（RSD=1.71%）.Condusion：This determination method is simple,accurate and reproducible,and suitable for the quality control of the medicinal materials and prepared slices of Rhizoma atractylodis.

关 键 词：气相色谱法 苍术 茅术醇 Β-桉叶醇 含量测定 

分 类 号：R917[医药卫生—药物分析学]