血清标记物B7-H4分子含量变化在卵巢癌诊断中的作用研究  

Research on the content changes of the serum markers B7-H4 molecules and its role in the diagnosis of ovarian cancer

作　　者：窦宇红[1] 吴雄君[1] 蒋艳[2] 

机构地区：[1]深圳市沙井人民医院,深圳518104 [2]南华大学生命科学院,衡阳421001

出　　处：《药物分析杂志》2010年第3期570-572,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:探讨新发现的血清标记物B7-H4在卵巢癌患者血清中的含量变化及其临床意义。方法:通过RT-PCR技术检测确诊为卵巢癌的35例患者及23例卵巢良性肿瘤患者的血清中B7-H4基因的mRNA含量变化,用ELISA夹心法检测其血清中的B7-H4水平变化,采用CA125II试剂盒检测所有患者CA125水平的变化,采用单因素方法分析比较B7-H4与CA125水平变化与患者病理类型的相关性;选取30例健康妇女血清作为对照。结果:RT-PCR检测结果发现卵巢癌患者B7-H4基因的mRNA含量明显高于正常组,P<0.05。与健康组与良性肿瘤组相比,卵巢癌患者血清中B7-H4的表达异常增多,P<0.05;其变化程度及准确率均高于CA125。结论:血清中B7-H4分子的含量变化可以作为卵巢癌诊断的一个重要的参考指标。Objective：To explore the change of content of the newly discovered serum markers B7-H4 in serum of ovarian cancer patients and its clinical significance. Methods：RT-PCR detection was used to detect the changes of mRNA content in B7-H4 gene in serum of 35 patients who were diagnosed as ovarian cancer and 23 cases of ovarian benign tumor, and sandwich ELISA assay was used to detect B7-H4 changes in the serum. CA125 II kits were taken to detect changes in the level of CA125 in all patients. Single-factor method was adopted to analyze and compare the relationship of the levels of B7-H4 and CA125 and the pathological types of patients; Serum of 30 cases of healthy women was selected as control. Results：Through the RT-PCR detection, mRNA content in B7-H4 gene were found significantly higher in ovarian cancer patients than in the control group, P 0.05. Compared with the healthy group and the benign tumor group, ovarian cancer patients have the unusual increase of B7-H4 expression, P 0.05; its change extent and accuracy were higher than CA125.Conclusion：The B7-H4 molecules content changes in serum can be used as an important indicator in the diagnosis of ovarian cancer.

关 键 词：卵巢癌 B7-H4 CA125 RT-PCR ELISA 

分 类 号：R917[医药卫生—药物分析学]