MICA外显子5多态性与成人迟发自身免疫型糖尿病的相关性研究  

The Aassociation of MICA Gene Exon 5 Polymorphism with Latent Autoimmune Diabetes in Adults

作　　者：刘鹏鹰[1] 丁洪光[2] 刘君英[1] 李瑛[1] 叶辉[1] 邬磊[1] 黄耀辉[1] 

机构地区：[1]深圳市第二人民医院,广东深圳518035 [2]广州医学院,广东广州510182

出　　处：《深圳中西医结合杂志》2010年第1期22-24,28,共4页Shenzhen Journal of Integrated Traditional Chinese and Western Medicine

基　　金：深圳市卫生局科技项目(A2001626)

摘　　要：目的:探讨MICA外显子5多态性与深圳成人迟发自身免疫性糖尿病(latent autoimmune diabetes in adults,LADA)的关系。方法:对21例LADA、28例2型糖尿病(T2DM)患者和31例正常人(NC)采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术和PAGE银染技术,对三组研究对象比较MICA外显子5多态性差异。结果:LADA患者A5.1等位基因频率显著高于正常对照组(35.7%vs.17.7%,=2.89,P<0.05);2型糖尿病MICA等位基因频率与正常组比较差异无统计学意义(P>0.05)。结论:MICA5.1等位基因与深圳市LADA易感性相关。Objective To investigate the association of MICA gene exon 5 polymorphism with latent autoimmune diabetes in adults in Shenzhen.Methods Alleles of MICA gene exon 5 was determined by PCR-RFLP and PAGE in 21 LADA patients, 28 type 2 diabetes mellitus patients and 31 healthy control subjects.Results A highly significant increase in the frequency of the A5.1 allele was seen in patients with LADA compared with that in controls（35.7% vs.17.7%, χ^2= 2.89 P〈0.05）.The A5.1 allele frequency was not significantly higher in patients with T2DM than in the control group（P〉0.05 ）.Conclusion The polymorphism of MICA gene confers susceptibility to LADA.

关 键 词：MICA基因外显子5 基因多态性 成人迟发自身免疫性糖尿病 

分 类 号：R587.1[医药卫生—内分泌]