内皮素-1在烫伤大鼠脑血管损伤中的表达  

Expression of endothelin-1 in cerebral vessels of scalded rats

作　　者：林雪群[1] 戴育成[2] 祝高春[1] 万丽丹[1] 

机构地区：[1]南昌大学医学院脑血管研究所,解剖学教研室,南昌330006 [2]南昌大学第二附属医院分子医学重点实验室,南昌330006

出　　处：《解剖学报》2010年第1期104-108,共5页Acta Anatomica Sinica

基　　金：江西省自然科学基金资助项目(0440062);江西省教育厅科技项目资助(赣教高字2007-6-80)

摘　　要：目的探讨内皮素-1(ET-1)在烫(烧)伤大鼠脑血管损伤的表达及其作用。方法应用组织学方法观察8只烫伤大鼠脑基底动脉组织结构的变化;应用RT-PCR测定48只烫伤大鼠脑基底动脉ET-1 mRNA的表达;应用Western blotting分析48只烫伤大鼠脑基底动脉ET-1的表达;应用放射免疫分析检测48只烫伤大鼠脑基底动脉ET-1水平的变化。结果烫伤大鼠脑基底动脉呈现病理学组织结构变化;烫伤后3h大鼠脑基底动脉ET-1 mRNA表达较正常对照组开始升高,6h达高峰;烫伤后6h大鼠脑基底动脉ET-1表达较正常对照组增强,12h达高峰;烫伤后各组大鼠脑基底动脉ET-1水平较正常对照组明显升高,6h达高峰。结论烫(烧)伤可诱发大鼠脑血管ET-1表达增加,增加表达的ET-1可能与烫(烧)伤大鼠脑血管损伤的病理发生有关。Objective To investigate the expression and effects of endothelin-1 （ET-1） in cerebral vessels of scalded rats. Method Histological method was used to observe the alteration of histological structure of basilar artery in 8 scalded rats. Reverse transcription polymerase chain reaction （RT-PCR） was performed to examine expression of ET-1 mRNA ; Western blotting to analyse expression of endothelin-1 ; radioimmunoassay to measure ET-1 levels, respectively, of cerebral basilar artery in 48 scalded rats. Result Vascular pathohistologieal changes were observed in cerebral basilar artery after rats were scalded; ET-1 , ET-1 mRNA expression and ET-1 levels of basilar artery in scald 6 hours, 12 hours and 24 hours group were obviously higher than that of control group. Conclusion Scald may cause an increases of ET-1 expression in basilar artery, and elevated ET-1 expression may relate to the pathogenesis of cerebral vascular injury in scalded rats.

关 键 词：内皮素-1 烫伤 脑动脉 免疫印迹法 反转录-聚合酶链反应 放射免疫分析 大鼠 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]