幽门螺杆菌提取液诱导AGS细胞内活性氧类物质水平的变化  

Changes of reactive oxygen species levels in the AGS cells induced by Itelicobacter pylori extracts

作　　者：黄学文 罗瑞华 赵琪 黄丽丽 安仙园 赵兰静 王玠 黄玉政[4] 

机构地区：[1]上海华东疗养院检验科,无锡214065 [2]上海华东疗养院胃镜室,无锡214065 [3]无锡人民医院检验科 [4]江苏省血吸虫病防治研究所

出　　处：《中华消化杂志》2010年第1期37-41,共5页Chinese Journal of Digestion

基　　金：上海市科委医学领域引导科技攻关资助项目（08411968100）

摘　　要：目的探讨幽门螺杆菌（Hp）感染与AGS细胞内活性氧类物质（ROS）的关系。方法将Hp11638（CagA^-，VacA^-）菌株及其突变株Hp11638M（CagA^＋，VacA^-）提取液与AGS细胞共同孵育，收集细胞及培养上清液。流式细胞术检测细胞内ROS水平，分光光度计550nm处检测培养上清液中细胞色素C含量。结果AGS细胞内ROS水平与2株Hp提取液呈浓度及时间依赖关系，经Hp11638提取液刺激的AGS细胞内ROS水平高于Hp11638M提取液。细胞色素C减少证实了这一结果。结论AGS细胞内ROS升高是Hp多种蛋白共同作用的结果，VacA蛋白参与了这一过程。Objective To investigate the relationship between Helicobacter pylori （H. pylori） infection and concentration of reactive oxygen species （ROS） in AGS cells. Methods AGS cells were cultured with either Hp11638 （CagA^＋, VacA^- ） extract or Hp11638 mutant （CagA^＋, VacA ^-） extract for 48 hours, then the cells and supernatants were collected. The concentration of ROS in AGS cells was measured by flow cytometry. The eytochrome C reduction was detected by spectrophotometer at 550 nm. Results The ROS levels in the AGS cells were correlated with two H. pylori strains in a concentration and time-dependent manner. The ROS levels in AGS cells treated with Hp11638 extract in different concentrations or times were correspondingly higher than those treated with Hp11638 mutant extract. Similar results were found in examination of cytochrome C reduction. Couelusion The elevation of ROS in AGS cells is related to effects of H. pylori proteins, and the VacA protein involves in the process.

关 键 词：幽门螺杆菌 VacA蛋白 CAGA蛋白 活性氧类物质 

分 类 号：R378[医药卫生—病原生物学]