利用帕金森症的果蝇模型测试具有神经保护作用的化学活性分子(英文)  

Evaluate Neuroprotective Chemicals in Drosophila Models of Parkinson's Disease

作　　者：乐志操[1] 黄爱玲[1] 杨宇丰[1] 

机构地区：[1]福州大学生命科学研究所,福州350108

出　　处：《中国生物化学与分子生物学报》2010年第2期128-133,共6页Chinese Journal of Biochemistry and Molecular Biology

摘　　要：帕金森症的果蝇模型对解析疾病的分子细胞机制贡献极大.为探讨利用地中海黑腹果蝇的疾病模型来筛选新型治疗帕金森症药物的可能性,我们构建了基于DJ-1A和PINK1两个遗传致病因子的帕金森症果蝇模型,测试抗氧化和消炎活性分子米诺环素和辅酶Q10对脑多巴胺浓度的影响.结果表明,米诺环素对DJ-1A果蝇模型有明显保护作用,能显著提高脑多巴胺的浓度,但是对PINK1果蝇模型没有保护作用;辅酶Q10对两种模型均有保护作用.因此,帕金森病的果蝇模型能够反映药物分子的特异性作用,为筛选新的帕金森病治疗药物提供了一条便捷的途径.The Drosophila model of Parkinson＇s disease has been invaluable for dissecting the molecular and cellular mechanism of the disease.To evaluate the possibility of utilizing Drosophila model in drug screening,two strains based on DJ-1A and PINK1 gene interference were constructed,and chemical molecules with anti-oxidant and anti-inflammatory property such as minocycline and coenzyme Q10 were tested.Brain dopamine levels were used as readout.We found that minocycline prevented brain dopamine level from declining in DJ-1A Drosophila model.This protective effect was not seen in PINK1 model.Coenzyme Q10 was protective in both models.The Drosophila model of Parkinson＇s disease revealed the specific function of chemicals,thus provided a novel platform to search for new drugs to treat the disease.

关 键 词：帕金森症 地中海黑腹果蝇 药物筛选 多巴胺 

分 类 号：Q78[生物学—分子生物学]