GluR2在大鼠下丘的年龄依赖性表达及其与突触发育的关系  

GluR2 expression in the developing rat inferior colliculus and the relationship with development of synapse

作　　者：沈宇玲[1] 金晓杰[1] 

机构地区：[1]上海交通大学医学院附属仁济医院耳鼻咽喉头颈外科,上海200001

出　　处：《临床耳鼻咽喉头颈外科杂志》2010年第4期173-176,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:检测α-氨基-3-羟基-5-甲基-4-异恶唑丙酸(AMPA)受体亚型GluR2及突触小泡蛋白(SYP)在不同年龄大鼠下丘中的表达,了解GluR2的年龄依赖性表达规律及其与突触发育的时间关系,以求对中枢神经系统听觉功能发育及可塑性的分子机制提供可能的实验依据。方法:用双重免疫荧光组织化学的方法研究大鼠生后发育过程中,GluR2及SYP在下丘中的表达规律及相互关系。结果:①GluR2在各周龄组大鼠下丘神经元中均有不同程度表达,生后2周表达较弱,3、4周表达渐强,6周时达高峰,与其他年龄组间差异有统计学意义,之后表达急剧下降,8、10周表达最弱,与其他年龄组间差异有统计学意义。②GluR2在大鼠下丘不同核团表达程度不同,中央核表达较强,外侧核和背侧核中表达较弱。③SYP在不同年龄大鼠下丘神经元中均有胞膜表达,2、3周表达较弱,4周时表达较强,6周为表达高峰,之后表达下降。6周与其他年龄组间差异有统计学意义,2、3、8、10周间差异无统计学意义。④大鼠生后下丘神经元中AMPA受体亚型GluR2的表达变化与突触小泡的数量改变趋势基本一致。结论:大鼠生后发育过程中下丘GluR2及SYP的表达呈现基本一致的年龄依赖性表达规律,说明GluR2在下丘的表达可能与下丘突触成熟及功能发育有关,其年龄依赖性表达现象可能是中枢神经系统感觉功能发育及可塑性的重要机制。Objective：To observe the GluR2 expression in rat inferior colliculus （IC） in different developmental stages ,and to investigate its developmental change and relationship with the synapse development.Method：The expression of GluR2 and synaptophysin（SYP） in IC were detected by double immunofluorescence method.Result：①All sorts of neurons in IC expressed GluR2 in every postnatal groups,and the GluR2 expression in P6w groups was higher than that in other groups.②The expression of GluR2 were different in different subnucleus of IC.③All sorts of neurons in IC expressed SYP in every postnatal groups,and the SYP expression in P6w groups was higher than others. ④The expressions of GluR2 consistented with the expression of SYP in IC.Conclusion：The developmental changes of GluR2 and SYP expression in the rat IC may be involved in the development and plasticity of auditory center.

关 键 词：α-氨基-3-羟基-5-甲基-4-异恶唑丙酸 突触小泡蛋白 下丘 

分 类 号：R764[医药卫生—耳鼻咽喉科]