急性肾衰竭家兔脾组织形态以及髓过氧化物酶和细胞膜泵活性的变化  

Changes of morphology and myeloperoxidase,membrane pump activities of spleen tissue in acute renal failure rabbits

作　　者：赵自刚[1] 刘军超[1] 张玉平[1] 侯亚利[1] 杜舒婷[1] 王小荣[1] 张静[1] 牛春雨[1] 

机构地区：[1]河北北方学院微循环研究所,河北张家口075029

出　　处：《中国病理生理杂志》2010年第3期509-512,共4页Chinese Journal of Pathophysiology

基　　金：河北省科技支撑计划资助项目(No.06276102D-17);河北省教育厅科研基金资助项目(No.2006306);张家口市科技局计划资助项目(No.061159)

摘　　要：目的:观察急性肾功能衰竭(ARF)家兔脾组织形态以及髓过氧化物酶(MPO)和细胞膜泵(ATPase)活性的变化,探讨脾在ARF免疫功能紊乱发病学中的作用。方法:42只家兔均分为对照组、HgCl2组、甘油组。其中HgCl2组以皮下注射1%HgCl2(1.3mL/kg)、甘油组以肌肉注射50%甘油(10mL/kg)分别复制ARF模型,均分为12h、24h、48h3个亚组。在不同时点,所有动物颈总动脉插管放血备检,测定血清反映肾功能的生化指标;制备脾组织切片,观察脾组织形态;并制备10%脾组织匀浆,检测MPO和ATPase活性。结果:模型组各时点的脾组织均有不同程度的淤血、脾小梁增多。HgCl2组与甘油组各时点的脾组织匀浆MPO活性均显著高于对照组,2组24h的MPO活性高于组内12h及48h;HgCl2组与甘油组在多个时点的Na+-K+-ATPase、Ca2+-ATPase、Mg2+-ATPase、Ca2+-Mg2+-ATPase活性均显著低于对照组,且随时间延长,各ATPase有逐渐降低的趋势,除甘油组的Mg2+-ATPase外,各组48h的ATPase均低于12h。结论:家兔ARF发展过程中,脾脏有组织学损伤,且存在中性粒细胞扣押、细胞膜泵活性降低,这可能是脾作为免疫器官导致ARF免疫功能紊乱的原因之一。AIM： To observe the changes of morphology, the activity of myeloperoxidase （MPO） and membrane pump activities of spleen tissue in acute renal failure （ARF） rabbits, and to inquire into the role of spleen on pathogenesis of immune function disorders during ARF. METHODS： 42 rabbits were divided into control group, HgCl2 group and glycerinum group. The ARF model was established by hypodermic injection of 1% HgCl2 at dose of 1.3 mL/kg in HgCl2 group, intramuscularly injection of 50% glycerinum at dose of 10 mL/kg in glycerinum group, respectively, and the animals were divided into the 12 h, 24 h, 48 h secondary groups （6 rabbits each group）. At different time points, the rabbits were cannulated to facilitate the collection of blood sample to examine the biochemical indexes of renal function. The spleen microscopic sections were prepared for observing the morphology. The spleen homogenate was made for determining the activities of MPO and membrane pumping. RESULTS： Pathological sections of spleen showed that the different degree of congestion was found and spleen trabecula was increased in two model groups at multiple-time points. The MPO activity of spleen homogenate in HgCl2 group and glycerinum group at all time points were obviously higher than that in control group, and at 24 h, the MPO activitie in two model groups was significantly increased than that in the same group at 12 h and 48 h. The activities of Na＋-K＋-ATPase, Ca2＋-ATPase, Mg2＋-ATPase, Ca2＋-Mg2＋-ATPase of spleen homogenate in two model groups at multiple time points were significantly lower than those in control group. Following ARF development, the ATPase activitie in two model groups at 48 h was lower than that at 12 h except the Mg2＋-ATPase in glycerinum group. CONCLUSION： Spleen as an immune organ has histological damage, arrest of polymorphonuclear neutrophils and dysfunction of membrane pump during the development of ARF in rabbits, leading to immune disorders.

关 键 词：肾功能衰竭 急性 脾 髓过氧化物酶 细胞膜泵 

分 类 号：R334.1[医药卫生—人体生理学] R363.2[医药卫生—基础医学]