高效液相色谱手性流动相添加剂法拆分头孢羟氨苄对映体  

Resolution of Enantiomers of Cefadroxil by HPLC using Mobile Phase with Added Chiral Additive

作　　者：苏立强[1] 高玉玲[1] 张晓林[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,齐齐哈尔161006

出　　处：《理化检验（化学分册）》2010年第3期273-275,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：黑龙江省科技厅科技攻关项目(GC02A313)

摘　　要：以羟丙基-β-环糊精(HP-β-CD)为手性流动相添加剂,提出了头孢羟氨苄对映体的高效液相色谱拆分方法。选用SinoChromODS-BP色谱柱(4.6mm×200mm,5μm),含16mmol.LHP-β-CD的甲醇-乙腈-水(10+10+80)混合溶液为流动相,pH为4.93,流速为0.30mL·min-1,紫外检测波长为232nm,进样量为10μL。试验结果表明:该法可使头孢羟氨苄的两对对映体得到良好的分离,分离度分别为1.51,1.53。Resolution of enantiomers of cefadroxil by HPLC was proposed by using the SinoChror ODS-BP chromatographic column （4. 6 mm×200 mm,5 μm）as stationary phase, and 16 mmol · L-1 hydroxypropyl -β- cyclodextrin （HP-β-CD） as a chiral agent added to the mobile phase which was a mixture of CH3 COH, CH3CN and H2O mixed in the ratio of 10＋10＋80. Acidity of the mobile phase was controlled at pH 4. 93 with a flow-rate of 0. 30 mL · min-1. UV detection at the wavelength of 232 nm was adopted for the determination, and volume of sample introducted was 10 μL. It was shown that the two pairs of enantiomers of cefadroxil were successfully separated by the proposed method and values of separation factor achieved were 1.51 and 1.53 respectively.

关 键 词：高效液相色谱 手性添加剂 头孢羟氨苄 对映体 羟丙基-Β-环糊精 

分 类 号：O652.63[理学—分析化学]