广东省O1/O139群霍乱弧菌耐药监测分析  

Surveillance of antibiotic susceptibility of Vibrio cholerae O1 and O139 in Guangdong province between 2006 and 2008

作　　者：李柏生[1] 谭海玲[1] 王多春[2] 邓小玲[1] 陈经雕[1] 柯碧霞[1] 柯昌文[1] 

机构地区：[1]广东省疾病预防控制中心微生物检验所,广东省应急病原学检测重点实验室,广州510300 [2]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室

出　　处：《中国公共卫生》2010年第3期321-322,共2页Chinese Journal of Public Health

基　　金：国家“863”高技术研究发展计划项目(2006AA02Z425);国家自然科学基金(30872260)

摘　　要：目的分析广东省霍乱病例及环境来源O1/O139群霍乱弧菌对抗生素的敏感性,为霍乱防控提供依据。方法选取2006-2008年广东省O1/O139群霍乱病例分离株38株,环境株145株,以毒素基因ctxAB的PCR检测区分产毒株与非产毒株;采用WHO推荐的改良Kirby-Bauer纸片法,分析霍乱弧菌对11种抗生素在体外的药物敏感性。结果183株O1/O139群霍乱弧菌中,ctxAB阳性44株,ctxAB阴性139株。分离自病例的菌株和ctxAB阳性菌株中,小川型菌株只对四环素、萘啶酸和复方新诺明有一定耐药;稻叶型菌株对萘啶酸和复方新诺明100%耐药,而O139群菌株对四环素、萘啶酸和复方新诺明等多种抗生素有一定耐药;分离自环境的菌株和ctxAB阴性菌株中,小川型和稻叶型菌株对四环素、萘啶酸和复方新诺明等多种抗生素有一定耐药,而O139群则仅对萘啶酸、复方新诺明和氨苄西林有一定耐药;分离自病例的菌株和ctxAB阳性菌株以耐多药为主,耐多药率分别为78.9%和75.0%,高于分离自环境的菌株和ctxAB阴性菌株的31.7%和30.9%(P<0.01)。结论广东省O1/O139群霍乱弧菌中分离自病例的菌株和ctxAB阳性菌株耐多药率较高,应予以密切关注。Objective To analyze antibiotic susceptibility of the V.cholerae strains isolated from clinical and environmental samples in Guangdong province during 2006 and 2008.Methods V.cholerae O1 and O139（183 strains） from cholera cases（38 strains） and environmental samples（145 strains） in Guangdong province during 2006 and 2008 were colected.The detection of ctxAB gene with PCR was employed to identify toxigenic and non-toxigenic strains.Antibiotic susceptibility testing for the strains was performed on Muller-Hinton agar with disk diffusion method as recommendid by CLSI（former NCCLS）.Results There were 44 ctxAB gene positive strains in the 183 strains.Among the clinical and toxigenic strains,serotype Ogawa showed resis-tance to tetracycline,nalidixic acid,and trimethoprim＋sulfamethoxazo;while the serotype Indba showed a resistance rate of 100% to nalidixic acid and trimethoprim＋sulfamethoxazo;the O139 showed resistance to tetracyc-line,nalidixic acid and trimethoprim＋sulfamethoxazol.The Ogawa and Inaba from environment and non-toxigenic serotype showed resistance to tetracycline,nalidixic acid and trimehtoprim＋sulfamethoxazol,and the O139 strains resisted to nalidixic acid,trimehtoprim＋sulfamethoxazol and ampicilin.Most of clinical and toxigenic strains showed stronger multidrug resistance than that of the environmental and non-toxigenic strains（P0.01）.The serotype Inaba from the clinical and toxigenic strains appeared NAL-SXT resistant,and most of the O139 showed multidrug resistance to more than 3 antibiotics.On the contrary,most of serotypes of Ogawa and Inaba from environmental and non-toxigenic strains showed multidrug resistance to more than 3 antibiotics,while the most of the O139 displayed resistant to NAL-AMP and SXT-AMP.Conclusion Most of clinical and toxigenic V.cholerae strains isolated in Guangdong province showed strong multidrug resistance than environmental and non-toxigenic strains.It is important to survey the multidrug resistance of the V.cholerae to control cholera effic

关 键 词：霍乱弧菌 药物敏感性试验 ctxAB毒素基因 耐药监测 

分 类 号：R378.3[医药卫生—病原生物学]