玉竹提取物A对小鼠免疫性肝损伤治疗作用的研究  

The Therapeutic Effects of EA-PAOA on Liver Injury Induced by Concanavalin A in Kunming Mice

作　　者：赵良中[1] 时文艳[1] 鞠晓红[1] 李妍[1] 

机构地区：[1]吉林医药学院病原教研室,吉林132013

出　　处：《中国畜牧兽医》2010年第3期160-163,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：本试验旨在研究玉竹提取物A(extraction A of polygonatum odoratum,EA-PAOA)对刀豆蛋白A(ConA)诱导的小鼠免疫性肝损伤的治疗作用,探讨其发挥此作用的可能机制。将昆明小鼠随机分成正常对照组、模型对照组、试验1组(EA-PAOA 0.5 g/kg)、试验2组(EA-PAOA 1 g/kg)、试验3组(EA-PAOA 2 g/kg)。除正常对照组外,其余各组均尾静脉注射20mg/kg的ConA 0.5 mL,每天06∶00注射1次,连续5 d。每天注射ConA 2 h后,EA-PAOA 3个试验组分别腹腔注射不同浓度的EA-PAOA 0.5 mL,每天3次,共4 d,第5天只在注射ConA后2 h注射1次EA-PAOA。末次注射EA-PAOA后6 h,摘眼球取血低温保存,待测谷丙转氨酶活性(ALT)、3个细胞因子;取肝待行肝脏组织病理学检查注射;取脾待行淋巴细胞增殖试验(MTT法)和流式细胞术以检测T淋巴细胞亚群。结果显示,EA-PAOA 3个试验组血清中,ALT、IFN-γ、TNF-α水平明显降低,IL-10水平明显升高,能显著抑制T淋巴细胞的转化增殖,与模型对照组相比差异极显著(P<0.01);但流式细胞术检测T淋巴细胞亚群发现,5组CD4+T和CD8+T淋巴细胞的数量和比例并无明显差别,都与正常对照组无显著差异;EA-PAOA 3个试验组肝脏组织病理改变较模型对照组明显改善。表明EA-PAOA可能是通过抑制T淋巴细胞的转化增殖,抑制其活性,调节Th1和Th2的数量及比例,降低血清中IFN-γ、TNF-α水平,提高IL-10水平,以此发挥其治疗免疫性肝损伤的功能。The objective of this paper was to investigate the therapeutic effects of EA-PAOA on the immune hepatic injury in Kunming mice and study its therapeutic mechanisms.Kunming mice were divided into five groups,which were normal control,model control,test 1（EA-PAOA 0.5 g/kg）,test 2（EA-PAOA 1 g/kg）,test 3（EA-PAOA 2 g/kg）.Model control and three test groups,respectively,received intravenous injections of Concanavalin A（20 mg/kg） 0.5 mL,once one day,five days in all.Two hours after the first injection of Concanavalin A,three test groups were administrated intraperitoneal injections of EA-PAOA 0.5 mL,three times one day,five days in all.Six hours after the last injection of EA-PAOA,blood were collected,the liver was reserved for routine light microscopy and the spleen was aimed to do T-cell proliferaction activity tests.We tested the effects of EA-PAOA on T lymphocyte subsets of spleen with flow cytometry.Compared to model control group,in the three EA-PAOA tests,the level of serum ALT,IFN-γ,TNF-α were lower,serum IL-10 were higher（P〈0.01）.EA-PAOA could obviously inhibit T-cell proliferactive activity.But the number of CD4＋ T and CD8＋ T lymphocyte in spleen with flow cytometry hadn＇t much difference.The results indicated that EA-PAOA had distinct anti-liver injury function.Therefore,we suppose that the therapeutic mechanisms of EA-PAOA could be to inhibit T-cell proliferaction activity,increase serum concentration of IL-10,and decrease serum concentration of IFN-γ,TNF-α.

关 键 词：免疫性肝损伤 刀豆蛋白A 玉竹提取物A 淋巴细胞转化增殖 

分 类 号：S853.74[农业科学—临床兽医学]