无机硅胶-有机聚合物混合基质C_4毛细管整体柱的制备和性能研究  

Preparation and Performance of Inorganic Silica Gel-Organic Polymer Mixture Based C_4 Capillary Monolithic Column

作　　者：吴琼[1] 王园朝[1] 金瑛芝[1] 

机构地区：[1]杭州师范大学材料与化学化工学院有机硅化学与材料技术教育部重点实验室,浙江杭州310036

出　　处：《杭州师范大学学报（自然科学版）》2010年第1期52-56,共5页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：浙江省自然科学基金(Y406237)

摘　　要：利用具有双功能基团的3-(三甲基氧基硅烷)丙基甲基丙烯酸酯在酸性水溶液中硅氧烷水解缩聚后,再在热引发条件下与甲基丙烯酸丁酯进行双键聚合反应,从而制备了一类新型的无机硅胶-有机聚合物混合基质毛细管电色谱整体柱,实现了硅胶基质整体柱和有机聚合基质整体柱制柱方法的合二为一.采用扫描电镜和氮吸附仪表征了整体柱固定相的微观结构.通过电压、缓冲溶液浓度、pH值和有机改性剂含量等因素对电渗流流速的影响,评价了整体柱的电色谱性能.利用制备柱基线分离了4种中性芳香烃类物质,结果表明该整体柱具有反相保留机理.H ydrolyzing 3-（trimethoxysilyl） propyl methacrylate （γ-MAPS） and co-condensation polymerizing in the presence of an acid solution, of which the production reacts with butyl methacrylate by double bond polymerization in the presence of a heat initiator, the paper has prepared a novel type of inorganic silica gel-organic polymer mixture based capillary monolithic column. The method has combined the preparation of silica-based monolithic column and organic polymer-based monolithic column. The paper has characterized the structure and pore size distribution of the monolithic material by scanning electronmicroscopy and nitrogen adsorption method, investigated the influences of running voltage, organic additive, concentration and pH of buffer on electroosmotic flow（EOF）, separated four neutral hydrocarbons, such as benzene, toluene, naphthalene, and biphenyl using the mixture based monolithic column. The results show that the monolithic column has a typical RP chromatographic retention mechanism.

关 键 词：整体柱 毛细管电色谱 无机硅胶-有机聚合物混合基质 分离 

分 类 号：O657.8[理学—分析化学]