与大豆孢囊线虫病抗性相关的RAPD标记  

RAPD Markers Associated with Resistance to Soybean Cyst Nematode

作　　者：章彦[1,2] 金芜军[1,2] 李莹[1,2] 刘学义[1,2] 方宣钧[1,2] 

机构地区：[1]中国农业科学院生物技术研究中心 [2]山西省农科院经济作物研究所

出　　处：《高技术通讯》1998年第11期45-48,共4页Chinese High Technology Letters

基　　金：863计划资助项目

摘　　要：利用ＲＡＰＤ－ＰＣＲ技术，对７个抗大豆孢囊线虫的大豆材料和１０个感病大豆品种的基因组ＤＮＡ进行了ＲＡＰＤ分析，供试的２００个随机引物均产生了清晰稳定的ＲＡＰＤ扩增产物，其中有２１个随机引物产生的ＲＡＰＤ扩增产物具有多态性。获得了５个与大豆孢囊线虫病抗性相关的ＤＮＡ片段，这些ＲＡＰＤ标记具有较高的重复性和稳定性，可辅助抗大豆孢囊线虫病大豆新品种的选育。RAPD PCR technology was employed to analyze genome DNA of 7 soybean accessions resistance to SCN and 10 soybean accessions sensitive to SCN. Two hundred random primers all got lucid and stable RAPD amplified products, and twenty one of these random primers had polymorphic products. Five DNA fragments showing significant relativity to the phenomenon of resistance to SCN were obtained. These RAPD markers have greater reproducibility and stablity and can assist to select new cultivaals resistant to SCN.

关 键 词：RAPD 大豆孢囊线虫 分子标记 抗病品种 育种 

分 类 号：S565.103.4[农业科学—作物学]