应用农杆菌转化水稻的研究  

Rice Transformation Mediated by Agrobacterium

作　　者：丁力[1] 丁月云[1] 

机构地区：[1]中国科学院遗传研究所

出　　处：《高技术通讯》1998年第11期49-52,共4页Chinese High Technology Letters

基　　金：863计划资助项目

摘　　要：利用农杆菌ＬＢＡ４４０４（ｐＴＯＫ２３３）转化水稻成熟胚愈伤组织，通过筛选获得抗性愈伤组织并再生出转化植株，转化频率为８．９％。转基因植株经分子生物学鉴定为阳性，外源基因ｇｕｓ在转化植株中表达亦提供了转化成功的证据。转基因植株的当代及Ｔ１代植株获得了种子。摸索了应用农杆菌转化水稻的方法、条件。利用干燥处理法抑菌并提高转化植株再生频率。Rice calli from mature embryos were transformed with Agrobacterium LBA4404(pTOK233). After selection and regeneration, transgenic plants were obtained. The frequency of transformation was 8.7%. PCR and Southern blot showed that the foreign genes had been transferred into plant chromosomes. X Gluc staining showed that foreign gene(gus) can express in plant cell. The seeds were obtained from transgenic plants and T 1 generation. Partial desiccation can inhibit Agrobacterium and increase the frequency of transformation. The methods were optimized, and the factors affecting transforming rice using Agrobacterium were studied.

关 键 词：水稻 农杆菌 转化植株 转基因水稻 

分 类 号：S511.032[农业科学—作物学]