濒危植物连香树居群的遗传多样性和遗传分化研究  

Genetic Diversity and Genetic Variation of Populations Endangered Tree Cercidiphyllum japonicum

作　　者：王静[1] 张小平[1] 李文良[1] 王磊[1] 吴建勋[1] 陈一锟[1] 

机构地区：[1]安徽师范大学安徽省重要生物资源保护与利用研究重点实验室安徽师范大学生物环境与生态安全安徽省高校重点实验室,芜湖241000

出　　处：《植物研究》2010年第2期208-214,共7页Bulletin of Botanical Research

基　　金：安徽省教育厅自然科学研究重点项目(KJ2007A092);国家教育部高等学校博士学科点专项科研基金(20060370001);安徽省高校自然科学研究重点项目(2006KJ061A);教育部科学技术研究重点项目(206066)

摘　　要：利用ISSR分子标记技术对濒危植物连香树10个居群的遗传多样性和遗传变异进行了分析,结果表明:连香树物种水平遗传多样性较高,多态位点百分率(PPB)达到69.59%,Nei’s基因多样性指数(H)和Shannon信息指数(I)分别为0.231 3和0.351 4;而在居群水平上,多态位点百分率(PPB)为30.61%,Nei’s基因多样性指数(H)和Shannon信息指数(I)分别为0.115 6和0.173 3。遗传变异分析表明,居群间遗传分化程度高,遗传分化系数(GST)为0.500 3,居群间基因流Nm为0.527 3。Mantel检测,居群间的遗传距离和地理距离之间不存在显著的相关性。生境的片断化使居群间的基因流受阻,可能是导致居群间高遗传分化和居群水平低遗传多样性的主要原因。By using inter-simple sequence repeat（ISSR） markers technique,this paper studied the genetic diversity and variation of ten populations of Cercidiphyllum japonicum.The results showed that at species level,the percentage of polymorphic loci（P） was 69.59%,Shannon＇s index（I） was 0.351 4 and Nei＇s gene diversity（H） was 0.231 3,indicating a high level of genetic diversity.Whereas,at population level,they were only 30.61%,0.173 3 and 0.115 6,respectively,suggesting a low level of genetic diversity.Genetic variation analysis showed that the coefficient of gene differentiation is 0.500 3 and the gene flow is 0.527 3 among populations,demonstrating a relatively high level genetic variation among the populations.Cluster analysis showed that there was no significant correlation between genetic distance and geographic distance.The high genetic variance among populations and the low genetic diversity within population could be attributed to the habitat fragmentation and the limited gene flow among populations.

关 键 词：濒危植物 连香树 遗传多样性 遗传分化 ISSR 

分 类 号：S567.19[农业科学—中草药栽培]