Nia1 and Nia2 are Involved in Exogenous Salicylic Acid-induced Nitric Oxide Generation and Stomatal Closure in Arabidopsis  

作　　者：Fushun Hao Shiling Zhao Huan Dong Huan Zhang Lirong Sun Chen Miao 

机构地区：[1]Henan Key Laboratory of Plant Stress Biology, College of Life Science, Henan University, Kaifeng 475004, China

出　　处：《Journal of Integrative Plant Biology》2010年第3期298-307,共10页植物学报（英文版）

基　　金：Supported by the National Natural Science Foundation ofChina (30670183);Henan Innovation Project Forb UniversityProminent Research Talents (2005KY(x010))

摘　　要：Phytohormone salicylic acid （SA） plays important roles in plant responses to environmental stress. However, knowledge about the molecular mechanisms for SA affecting the stomatal movements is limited. In this paper, we demonstrated that exogenous SA significantly induced stomatal closure and nitric oxide （NO） generation in Arabidopsis guard cells based on genetic and physiological data. These effects were significantly inhibited by the NO scavenger c-PTIO, NO synthase （NOS） inhibitor L-NAME or nitrate reductase suppressor tungstate respectively, implying that NOS and nitrate reductase （NR） participate in SA-evoked stomatal closing. Furthermore, the effects of SA promotion of stomatal closure and NO synthesis are significantly suppressed in NR single mutants of nial, nia2 or double mutant niallnia2, compared with the wild type plants. This suggests that both Nial and Nia2 are involved in SA-stimulated stomatal closure. In addition, pharmacological experiments showed that protein kinases, cGMP and cADPR are involved in SA-mediated NO accumulation and stomatal closure induced by SA in Arabidopsis.Phytohormone salicylic acid （SA） plays important roles in plant responses to environmental stress. However, knowledge about the molecular mechanisms for SA affecting the stomatal movements is limited. In this paper, we demonstrated that exogenous SA significantly induced stomatal closure and nitric oxide （NO） generation in Arabidopsis guard cells based on genetic and physiological data. These effects were significantly inhibited by the NO scavenger c-PTIO, NO synthase （NOS） inhibitor L-NAME or nitrate reductase suppressor tungstate respectively, implying that NOS and nitrate reductase （NR） participate in SA-evoked stomatal closing. Furthermore, the effects of SA promotion of stomatal closure and NO synthesis are significantly suppressed in NR single mutants of nial, nia2 or double mutant niallnia2, compared with the wild type plants. This suggests that both Nial and Nia2 are involved in SA-stimulated stomatal closure. In addition, pharmacological experiments showed that protein kinases, cGMP and cADPR are involved in SA-mediated NO accumulation and stomatal closure induced by SA in Arabidopsis.

分 类 号：Q559[生物学—生物化学] Q945.172