肿瘤坏死因子-α基因多态性与煤肺遗传易感性关系的研究  

The Association of Tumor Necros is Factor-agene Polymorphisms With the Susceptibil ity to Coalworker's lung

作　　者：孙丽华[1] 尹建新 乌兰[3] 姜丽萍[4,5] 

机构地区：[1]内蒙古医学院第一附属医院神经内科,内蒙古呼和浩特010050 [2]内蒙古卫生厅监督局,内蒙古呼和浩特010055 [3]内蒙古医学院第一附属医院职业病科,内蒙古呼和浩特010050 [4]内蒙古医学院 [5]内蒙古自治区医院,内蒙古呼和浩特010059

出　　处：《疾病监测与控制》2010年第3期129-131,共3页Journal of Diseases Monitor and Control

基　　金：内蒙古医学院青年基金NY2006QN004

摘　　要：为研究肿瘤坏死因子(TNF)-α基因启动子区-308和-238位点G→A的碱基突变与中国内蒙古地区汉族人群煤肺遗传易感性的相关性。选择69名汉族男性煤肺患者为病例组,125名煤尘接触者为对照组,填写统一的调查问卷、拍摄高仟伏X射线后前位胸片、采集外周静脉血,采用多聚酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测TNF-α-308和-238位点基因多态性。结果表明:煤肺组等位基因TNF-α-308A的分布频率均显著高与对照组(P<0.05);TNF-α-238位点煤肺组等位基因A的分布频率,远高于对照组(P<0.05)。因此,TNF-α-308及-238位点基因G→A的碱基突变与中国内蒙古地区汉族人煤肺的发生及其严重程度有关,TNF-α可能影响煤肺患者的转归和存活。to study on the relationship between the -308 and -238 locus（G→A）mutation within the tumornecrosis factor-αgene（TNF-α）promoter region and the susceptibility to coalworker＇s lung in the Han population of Inner Mongulia China.Governed by the principles of voluntatiness and cooperation,75 patients with coalworker＇s lung and 137 controls with coal-exposure but without coalworker＇s lung were recruited.Each person were asked to fill a uniformed questionnaire.A chest high-voltage X-ray film was taken for each one.Blood sample from each one was analyzed using the polymerase chain reaction restriction fragment length polymorphisms（PCR-RFLP） technique for gene polymorphisms of TNF-α-308 and -238.The result showed that the frequencies of TNF-α-308A in the 75 patients with coalworker＇s lung were higher than those in the 137 controls（P0.05）.And in the coalworker＇s lung group,the frequencies of TNF-α-238 were significantly higher than control group.While the TNF-α-308 and -238 gene polymorphisms with the specific GA genotype demonstrated coalworker＇s lung risk reduction.

关 键 词：肿瘤坏死因子-Α 基因多态性 煤肺 

分 类 号：R33-33[医药卫生—人体生理学]