α-硫辛酸对高糖诱导的血管内皮细胞凋亡的保护作用  

The protective role of α-lipoic acid in the high glucose-induced apoptosis of vascular endothelial cells

作　　者：张军霞[1] 向光大[1] 孙慧伶[1] 赵林双[1] 侯洁[1] 乐岭[1] 翟振燕[1] 

机构地区：[1]广州军区武汉总医院内分泌科,湖北430070

出　　处：《中国糖尿病杂志》2010年第3期214-216,共3页Chinese Journal of Diabetes

基　　金：湖北省科学技术研究与开发资金项目(2009CDB427)

摘　　要：目的观察α-硫辛酸对高糖诱导的血管内皮细胞凋亡是否有保护作用。方法以不同浓度α-硫辛酸干预高糖作用下的血管内皮细胞(ECV304),共同孵育72h,以Annexin-FITC/PI双染流式细胞术和TUNEL法检测细胞凋亡。结果高糖明显增加内皮细胞凋亡(P<0.01);以不同浓度α-硫辛酸干预,内皮细胞凋亡明显减少(P<0.01),这种作用具有浓度依赖性。结论α-硫辛酸对高糖诱导的血管内皮细胞凋亡有保护作用。Objective To investigate the role of a-lipoic acid in the high glucose-induced apoptosis of vascular endothelial cells. Methods Human umbilical vein endothelial cells（ECV304）were incubated for 72h with high glucose plus α-lipoic acid in a series of concentrations. Apoptosis of ECV304 was observed by flow cytometry using Annexin-FITC/propidiumiodid （PI） staining and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling （TUNEL） assay. Results High glucose increased the apoptotic percentage of ECV304 significantly（P〈0. 01）, but the apoptosis of ECV304 incubated with high glucose which was interfered by α-lipoic acid in a series of concentrations decreased significantly in a dosedependent manner（P〈 0.01）. Conclusions wlipoic acid has a protective effect on the high glucoseinduced apoptosis of vascular endothelial cells.

关 键 词：葡萄糖 Α-硫辛酸 内皮细胞 凋亡 

分 类 号：R587[医药卫生—内分泌]