毛细管电泳-安培检测法测定中药四季青中的6种活性成分  

Determination of Six Kinds of Active Ingredients of Si Ji Qing by Capillary Electrophoresis With Amperometric Detection

作　　者：唐祝兴[1] 张东瑾 王巧玲[3] 

机构地区：[1]沈阳理工大学环境与化学工程学院,辽宁沈阳110159 [2]沈阳市化工学校医药系,辽宁沈阳110122 [3]沈阳医学院,辽宁沈阳110034

出　　处：《辽宁化工》2010年第3期332-335,共4页Liaoning Chemical Industry

基　　金：沈阳理工大学博士启动资金

摘　　要：采用毛细管电泳-安培检测法(CE-AD),同时测定了中药四季青的主要活性成分异香草酸、龙胆酸、山奈酚、槲皮素、咖啡酸和原儿茶酸。考察了工作电极电位、pH值、运行缓冲液的浓度、分离电压和进样时间的影响。在最佳的试验条件下,50 mmol/L硼砂缓冲溶液(pH=9.0)为运行缓冲溶液。工作电极为直径300 m的碳圆盘电极,检测电位为+0.95 V(vs.SCE)。被测物的线性范围超过3个数量级。该方法成功的被应用于实际样品的分析,获得了令人满意的结果。A method based on capillary electrophoresis with electrochemical detection has been developed for separation and de- termination of isovanillicacid, gentisic acid, kaempferol, quercetin, caffeic acid and protocatechuic acid in Si Ji Qing. The effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time were investigated. Under the optimum conditions, the analytes could be separated in a 50 mmol/L borate buffer（ pH 9.0）. A 300 μm diameter carbon disk electrode has a good response at ＋ 0.95 V （ versus SCE） for all analytes. The response was linear over three orders . The method has been successfully applied to the analysis of real sample, with satisfactory results.

关 键 词：毛细管电泳 安培检测 四季青 

分 类 号：O657.8[理学—分析化学]