接种AMF对葡萄微繁苗生长效应的研究  

Study on the Effects of AMF on the Growth and Development of Micropropagated Grape Plantlets

作　　者：谢丽源[1,2] 张勇[3,2] 熊丙全[2] 曾明[2] 

机构地区：[1]四川省农业科学院土壤肥料研究所,四川成都610066 [2]西南大学研究生部,重庆400716 [3]电子科技大学生命科学与技术学院,四川成都610054

出　　处：《北方园艺》2010年第5期18-23,共6页Northern Horticulture

基　　金：国家科技攻关资助项目(2001BA604A05);重庆市应用基础研究资助项目(20001095)

摘　　要：以葡萄为试验材料,在练苗期间接种AMF孢子菌剂,研究微繁苗移栽过程中AMF侵染过程,分析菌根化进程中微繁苗根系活力的变化以及AM共生体对微繁苗生长发育的促进效应。结果表明:AMF对葡萄微繁苗的生长发育具有重要促进作用,能够促进根系活力,微繁苗根系磷酸酶活性与AMF侵染率存在显著相关性。接种AMF可增强幼苗根系活力,促进根系对N、P等矿质养分的吸收和积累,并促进植株的光合作用,提高幼苗的生长势。In this experiment, the effects of inoculation with AMP on the growth and acclimatization of micropropagated plants during ex vitro transfer were investigated. The results showed that AMF had importantly stimulative effects on micropropagated grape plantlets in in vitro and ex vitro condition. The activity of phosphatase in micropropagated grape plantlets root was distinctly enhanced by AMF infection, at the same time, there was significant correlation between the activity of phosphatase and AMF infection rate. Inoculation with the AMF improved formation of a well-developed root system that was converted into an AM system. Introduction of the AMF at the time plantlets were transferred from in vitro conditions to ex vitro conditions improved the absorbency of root, shoot and root growth, increased the root/shoot ratio and the content of N, P in plant tissues, promoted photosynthesis and growth potential, and helped plantlets to tolerate environmental stress at transplanting. Thus, AM formation was seemed to be the key factor for subsement growth and development of micropropagated plantlets.

关 键 词：葡萄 微繁苗 丛枝菌根真菌 生长效应 

分 类 号：S663.103.6[农业科学—果树学]