蜜环菌对镁的耐性和富集特性  

Magnesium Tolerance and Enrichment Characteristics of Armillariella mellea

作　　者：朱林[1] 程显好[1] 冯志彬[1] 屈慧鸽[1] 图力古尔[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《微生物学通报》2010年第3期362-368,共7页Microbiology China

基　　金：食用菌技术"泰山学者"建设工程专项经费资助项目(No.200805);鲁东大学人才基金资助项目(No.LY20063304);鲁东大学食用菌技术创新团队基金资助项目

摘　　要：研究了镁对蜜环菌生长的影响,蜜环菌对于镁的耐性和富集规律,以及高浓度镁胁迫下蜜环菌的抗氧化酶的变化情况。3-15g/L的Mg浓度对于蜜环菌菌体的生长有促进作用。Mg浓度19g/L以上时,蜜环菌菌体的生长受到抑制。蜜环菌的子实体形成和子实体生物量在Mg的浓度为11g/L以下时不受影响,超过11g/L则子实体不能萌发。皮壳状菌丝和菌索中Mg的含量随培养基中Mg浓度的增大而增大,培养基Mg浓度达到16g/L后,菌丝、菌索中Mg的含量都不再上升。子实体对Mg的富集量比菌丝体小的多,在培养基Mg浓度在9、10g/L时,子实体中Mg的含量与对照组有显著差异。随着培养基中Mg浓度的提高,菌丝和菌索POD、CAT、SOD活性都有增加,而且菌丝与菌索之间抗氧化酶活力的差异随着培养基中Mg浓度的提高而增大。The impact of magnesium on growth,magnesium tolerance and enrichment features,as well as changes in activity of antioxidant enzymes under high-magnesium-concentration stress in Armillariella mellea were investigated.Magnesium at concentrations between 3-15 g/L in growth media promoted the growth of mycelia of A.mellea,but inhibited the growth at concentrations above 19 g/L.The formation and biomass production of fruiting body were not affected when magnesium concentration was below 11 g/L.At magnesium concentrations above 11 g/L the fruiting body formation stopped.The Mg content in crustose aerial hypha and rhizomorphs of A.mellea increased with increasing Mg concentration in growth medium up to a concentration of 16 g/L,but ceased to increase when the Mg concentration in medium was over 16 g/L.The Mg content in fruiting body of A.mellea grown on medium containing 9 or 10 g/L Mg was significantly different from that on control medium.The activity of antioxidant enzymes including peroxide （POD）,catalase （CAT） and superoxide dismutase （SOD） in crustose aerial hypha and rhizomorphs of A.mellea,as well as the differences in the activity of antioxidant enzymes increased with increasing Mg concentration in growth medium.

关 键 词：子实体 菌丝体 菌索 抗氧化酶 

分 类 号：S567.39[农业科学—中草药栽培]