A novel marker for the platyfish (Xiphophorus maculatus) W chromosome is derived from a Polinton transposon  

作　　者：Qingchun Zhou Ingo Braasch Alexander Froschauer Astrid Boehne Christina Schultheis Manfred Schartl Jean-Nicolas Volff 

机构地区：[1]Biofuture Research Group, Physiologische Chemie I, Biozentrum, University ofWiirzburg, Wiirzburg 97074, Germany [2]Hubei Key Laboratory of Genetic Regulation and lntegrative Biology, College of Life Science, Huazhong Normal University, Wuhan 97074, China [3]Institut fur Zoologie, Technische Universitait Dresden, Dresden 01062, Germany [4]Institut de Genomique Fonctionnelle de Lyon, Universit~ de Lyon, Institut Fdddratif Biosciences Gerland Lyon Sud, UniversiM Lyon 1, CNRS, INRA, Ecole Normale Supdrieure de Lyon, Lyon 69364, France

出　　处：《Journal of Genetics and Genomics》2010年第3期181-188,共8页遗传学报（英文版）

基　　金：supported by the grants from the Biofuture Programme of the German Federal Ministry for Education and Research (BMBF,to JNV),the French Research Agency (ANR to JNV);the Scientific Research Foundation for the Returned Overseas Chinese Scholars,the Ministry of Education of China (to QZ)

摘　　要：A consensus sequence, encoding a putative DNA polymerase type B derived from a Polinton transposon, was assembled from the sex determination region of Xiphophorus maculatus. This predicted protein, which is 1,158 aa in length, contains a DNAA_pol_B_2 domain and a DTDS motif. The DNA polymerase type B gene has about 10 copies in the haploid X. maculatus genome with one Y-specific copy. Interestingly, it has specific copies on the W chromosome in the X. maculatus Usumacinta strain （sex determination with female het- erogamety）, which represent new markers for this type of sex chromosome in platyfish. This marker with W- and Y-specific copies suggests relationship between different types of gonosomes and allows comparing male and female heterogameties in the platyfish. Further molecular analysis of the DNA polymerase type B gene in X. maculatus will shed new light on the evolution of sex chromosomes in platyfish.A consensus sequence, encoding a putative DNA polymerase type B derived from a Polinton transposon, was assembled from the sex determination region of Xiphophorus maculatus. This predicted protein, which is 1,158 aa in length, contains a DNAA_pol_B_2 domain and a DTDS motif. The DNA polymerase type B gene has about 10 copies in the haploid X. maculatus genome with one Y-specific copy. Interestingly, it has specific copies on the W chromosome in the X. maculatus Usumacinta strain （sex determination with female het- erogamety）, which represent new markers for this type of sex chromosome in platyfish. This marker with W- and Y-specific copies suggests relationship between different types of gonosomes and allows comparing male and female heterogameties in the platyfish. Further molecular analysis of the DNA polymerase type B gene in X. maculatus will shed new light on the evolution of sex chromosomes in platyfish.

关 键 词：DNA polymerase type B chromosome marker Xiphophorus maculatus 

分 类 号：Q78[生物学—分子生物学] S881.2[农业科学—特种经济动物饲养]