基于花瓣的傅里叶变换红外光谱鉴别分类杜鹃属植物  

Differentiation of Rhododendron Based on the Infrared Spectra of Petals

作　　者：罗庇荣[1] 刘刚[1] 张玉宾[1] 周湘萍[1] 桑林[2] 

机构地区：[1]云南师范大学物理与电子信息学院,云南昆明650092 [2]云南师范大学生命科学院,云南昆明650092

出　　处：《光谱学与光谱分析》2010年第4期943-948,共6页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(10764006);云南省自然科学基金项目(2007A048M)资助

摘　　要：利用傅里叶变换红外光谱（FTIR）技术结合适当的方法对杜鹃属植物不同亚属进行鉴别分类研究。测试了4个亚属植物花瓣的红外光谱，结果显示同种植物花瓣的红外图谱，峰形相似；不同种植物花瓣的红外光谱图在混合模区（1500～1200cm^-1）略有差异。为了进一步快速对其分类群进行鉴别，利用OMNIC软件分层抽样建立3个光谱数据库，未知样品光谱分别与光谱数据库比较得出匹配数值进行鉴别。研究结果显示此方法能够准确地鉴别分类4个亚属植物，这为大型的植物系统分类研究提供了一种潜在的方法。Several techniques were used to identify and classify plants. Mid-infrared spectroscopy combined with appropriate software was used in an attempt to differentiate different subgenus from Rhododendron. Fourier transform infrared （FTIR） spectroscopy was used for obtaining vibrational spectra of 46 petals from Rhododendron. Very minor differences were observed in the FTIR spectra among four subgenuses. For the purpose of rapid differentiation, libraries of spectra were created using samples from each subgenus variety. Spectra of unknown samples were recorded and compared with those of the libraries and the rate of affinity （the match value） was measured automatically using the appropriate software （OMNIC）. The results showed that petal samples from different subgenus varieties can be differentiated from each other. The study demonstrates that combining FTIR spectroscopy with appropriate analysis method can classify Rhododendron plants at subgenus level. It offers a potential method for the taxonomic research on plants system.

关 键 词：傅里叶变换红外光谱 杜鹃花属 亚属 鉴别分类 

分 类 号：O657.3[理学—分析化学]