用原子吸收光谱法测定水稻白叶枯病菌rpfFxoo基因缺失突变体铁元素吸收  

Ferric Iron Absorption in ΔrpfFxoo,a Gene Deletion Mutant of Xanthomonas oryzae pv.Oryzae,Assayed Using Atomic Absorption Spectrophotometry

作　　者：孙蕾[1] 吴茂森[1] 何晨阳[1] 

机构地区：[1]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《光谱学与光谱分析》2010年第4期1117-1119,共3页Spectroscopy and Spectral Analysis

基　　金：中央财政国家重点实验室自主研究课题专项(SKL2007SR06)资助

摘　　要：铁元素吸收是细菌生长的重要限制因素之一。前期研究推测在水稻白叶枯病菌(Xanthomonasoryzaepv.oryzae,简称Xoo)中rpfFxoo基因可能参与Fe元素的代谢。通过同源重组法,获得了Xoo基因缺失突变体ΔrpfFxoo;利用原子吸收光谱法测定Xoo野生型菌株PXO99A和ΔrpfFxoo对Fe元素的吸收。结果表明,通过对工作条件的优化,使样品平均回收率达到99.7%,相对标准偏差(RSD)为1.89;随着培养时间的延长,PXO99A和ΔrpfFxoo对Fe元素吸收不断增加,ΔrpfFxoo中Fe元素的含量显著低于PXO99A(P<0.05),证明rpfFxoo与铁元素的代谢有关。The ferric iron absorption is one of the most important limiting factors of bacterial growth of Xanthornonas oryzae pv. oryzae. It has been previously speculated that rpfFxoo might be involved in the ferric iron metabolism of the pathogen. In the present study, △rpfFxoo, a gene deletion mutant, was generated from the wild-type strain PXO99A of Xoo through the homologous recombination, and Fe content was assayed using flame atomic absorption in PXO99A and △rpfFxoo. The results indicated that the recovery was 99.7% and the relative standard deviation was 1.89 under optimized AAS operating conditions. The increase in Fe absorption in PXO99A and △rpfFxoo was observed with the increasing time. However, the ferric content of △rpfFxoo was significantly lower than that of PXO99A（P〈0. 05）. It is suggested that rpfFxoo is involved in iron metabolism in Xanthomonas oryzae pv. oryzae.

关 键 词：水稻白叶枯病菌 ΔrpfFxoo 铁元素吸收 原子吸收光谱法 

分 类 号：O657.3[理学—分析化学]