基于体外消化模型的香蕉果肉中可溶态Ca含量分析  

Analysis of Soluble Ca Content in Banana Pulp Based on Pretreatment of Digestion Model in Vitro

作　　者：李积华[1,2,3] 黄茂芳[1,2,3] 徐飞[4] 唐永富[1,2] 付调坤[1] 

机构地区：[1]中国热带农业科学院农产品加工研究所,广东湛江524001 [2]农业部热带作物产品加工重点开放实验室,广东湛江524001 [3]国家重要热带作物工程技术研究中心,海南海口571700 [4]海南大学,海南儋洲571737

出　　处：《光谱学与光谱分析》2010年第4期1120-1122,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(50763006);海南省自然科学基金项目(50890);农业部热带作物产品加工重点开放实验室科技基金项目(807032)资助

摘　　要：在香蕉果肉可溶态Ca含量分析过程中,引入体外消化模型进行预处理,采用AA700型火焰原子吸收分光光度计测量Ca含量。结果表明:(1)八成熟香蕉果肉中Ca含量为1.479 mg.g-1,平行分析实验结果RSD为4.12%;(2)八成熟香蕉果肉经过体外消化模型处理后,可溶态和不溶态Ca含量分别为1.108和0.412 mg.g-1,Ca溶解率和残留率分别为74.9%和27.8%,Ca主要以可溶态形式存在;(3)Ca溶解率和残留率平行实验结果的RSD分别为2.56%和9.10%,说明体外消化模型的引入并没有对实验方法精密度产生显著影响,且溶解率和残留率之和与理论值的误差仅为2.7%,进一步说明在食品微量元素形态分离分析过程中引入体外消化模型可行;(4)Ca测试方法的精密度和回收实验表明,样品回收率为99.2%,RSD为0.11%(n=9)。In the present paper, soluble Ca content in banana pulp was analyzed with pretreatment of digestion model in vitro. All the samples were digested by strong acid （perchloric acid and nitric acid with 1 ： 4 ratio） and determined by AA700 flame atomic absorption spectrophotometry （FAAS）. Meanwhile, the reproducibility of the analytical method was investigated. The resuits showed that （1） Content of Ca was 1. 479 mg ·g^-1 . DW in eighty percent maturity banana pulp. And the relative standard deviation was 4. 12%. （2） Contents of Ca in the soluble and insoluble fractions were 1. 108 and 0. 412 mg·g ^-1, respectively in eighty percent maturity banana pulp with pretreatment of digestion model in vitro. The extraction rate was 74.9% and the residue rate was 27.8% （3） The relative standard deviations of the analytical results of Ca contents in these two fractions were 2. 56% and 9.10%, respectively, in the reproducibility tests. It was showed that the digestion model in vitro did not affect the reproduction quality significantly. Moreover, the deviation between the theoretical value and the summation value of the ex traction and the residue rates was only 2.7%, which showed good availability of the digestion model in vitro to be used as a pretreatment method in mineral element analysis process. （4） Relative standard deviation and recovery yield in the determination procedure were 0. 11%（n=9） and 99.2%, respectively.

关 键 词：微量元素 香蕉 体外消化模型 

分 类 号：O657.3[理学—分析化学]