FQ-PCR技术在婴幼儿沙眼衣原体肺炎早期诊断中的应用  

Early diagnosis of infant chlamydia trachomatis pneumonia by FQ-PCR technique

作　　者：李基明 张慧芬 

机构地区：[1]福建省厦门市妇幼保健院,福建厦门361000

出　　处：《中国卫生检验杂志》2010年第2期325-326,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的：探讨荧光定量PCR技术（FQ-PCR）在婴幼儿沙眼衣原体肺炎早期诊断中的应用。方法：将2007年1月-2009年4月份收治的986例0-3岁婴幼儿肺炎作为研究对象,根据年龄分组,对各组婴幼儿均通过咽拭子获取标本,进行沙眼衣原体DNA（CT-DNA）测定;总结分析CT-DNA阳性病例。结果：986例标本中,CT-DNA阳性64例;其中3个月以内婴儿CT肺炎检出率最高,达13.8%,CT肺炎往往为无发热性肺炎,常伴有结膜炎是其特征,而X线多表现为支气管肺炎,其临床表现无特异性。结论：CT是婴幼儿肺炎的病原体之一;CT肺炎在婴幼儿3个月内检出率最高;FQ-PCR法是检测CT-DNA最直接、可靠的方法。Objective：To explore FQ-PCR technique to check infant chlamydia trachomatis pneumonia.Methods：Selecting 964 cases infant pneumonia and grouping them by ages,all cases were checked CT-DNA by pharyngal specimen and analyse positive cases.Results：There are 64 positive cases.The detection rate of CT pneumonia is 13.8% among less than 3 month infant,a few of them have a fever,a lot of them have a bronchial pneumonia and conjunctivitis.The clinical exhibitions are not unique.Conclusion：CT is a pathogen of infant pneumonia.The detection rate of CT pneumonia is maximal among less than 3 month infant.FQ-PCR is the best method to check CT-DNA.

关 键 词：沙眼衣原体 荧光定量PCR 婴幼儿肺炎 

分 类 号：R446.5[医药卫生—诊断学]